RCN3 Protein (AA 21-328) (His tag)
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- Target See all RCN3 Proteins
- RCN3 (Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-328
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This RCN3 protein is labelled with His tag.
- Purpose
- Recombinant Human Reticulocalbin-3/RCN3 (C-6His)
- Sequence
- KPSPDAGPHG QGRVHQAAPL SDAPHDDAHG NFQYDHEAFL GREVAKEFDQ LTPEESQARL GRIVDRMDRA GDGDGWVSLA ELRAWIAHTQ QRHIRDSVSA AWDTYDTDRD GRVGWEELRN ATYGHYAPGE EFHDVEDAET YKKMLARDER RFRVADQDGD SMATREELTA FLHPEEFPHM RDIVIAETLE DLDRNKDGYV QVEEYIADLY SAEPGEEEPA WVQTERQQFR DFRDLNKDGH LDGSEVGHWV LPPAQDQPLV EANHLLHESD TDKDGRLSKA EILGNWNMFV GSQATNYGED LTRHHDELVD HHHHHH
- Characteristics
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Recombinant Human Reticulocalbin-3/RCN3 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product RCN3 Protein
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Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3) (AA 21-328) protein (His tag)
RCN3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3) protein (His tag)RCN3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3) protein (Myc-DYKDDDDK Tag)RCN3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM DTT,10 % Glycerol, pH 7.4.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- RCN3 (Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3))
- Alternative Name
- Reticulocalbin-3 (RCN3 Products)
- Synonyms
- RLP49 Protein, 6030455P07Rik Protein, D530026G20Rik Protein, D7Ertd671e Protein, reticulocalbin 3 Protein, reticulocalbin 3, EF-hand calcium binding domain Protein, RCN3 Protein, Rcn3 Protein
- Background
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Recombinant Human Reticulocalbin-3/RCN3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Lys21-Leu328) of Human RCN3 fused with a polyhistidine tag at the C-terminus.
Reticulocalbin-3 is a member of the CREC family that is involved in the secretory pathway. Members of the CREC family consist of a number of multiple EF-hand domains. Reticulocalbin-3 localizes to the endoplasmic reticulum lumen. The bioinformaticanalysis of Reticulocalbin-3 reveal that it is a putative Ca2+-binding protein. In addition, it has been shown that autoactivation and secretion of PACE4 was increased upon co-expression with Reticulocalbin-3. The proPACE4-RCN-3 complex is plays an important role in the biosynthesis of PACE4. - Molecular Weight
- 36.2 kDa
- UniProt
- Q96D15
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