CGREF1 Protein (AA 20-301) (His tag)

Catalog No. ABIN1691476

Product Details

Target: CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))

Protein Type: Recombinant

Protein Characteristics: AA 20-301

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CGREF1 protein is labelled with His tag.

Purpose: Recombinant Human CGREF1/CGR11 (C-6His)

Sequence: APKDGVTRPD SEVQHQLLPN PFQPGQEQLG LLQSYLKGLG RTEVQLEHLS REQVLLYLFA LHDYDQSGQL DGLELLSMLT AALAPGAANS PTTNPVILIV DKVLETQDLN GDGLMTPAEL INFPGVALRH VEPGEPLAPS PQEPQAVGRQ SLLAKSPLRQ ETQEAPGPRE EAKGQVEARR ESLDPVQEPG GQAEADGDVP GPRGEAEGQA EAKGDAPGPR GEAGGQAEAE GDAPGPRGEA GGQAEARENG EEAKELPGET LESKNTQNDF EVHIVQVEND EIVDHHHHHH

Characteristics: Recombinant Human Cell Growth Regulator with EF Hand Domain Protein 1/CGREF1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala20-Ile301) of Human CGREF1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM GaCl2, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))

Alternative Name: CGREF1 (CGREF1 Products)

Synonyms: CGR11 Protein, CGREF1 Protein, 1110004G24Rik Protein, AW045201 Protein, Cgr11 Protein, cell growth regulator with EF-hand domain 1 Protein, cell growth regulator with EF-hand domain 1 L homeolog Protein, cell growth regulator with ring finger domain 1 Protein, cell growth regulator with EF hand domain 1 Protein, CGREF1 Protein, cgref1.L Protein, cgref1 Protein, CGRRF1 Protein, Cgref1 Protein

Sub Type: Fusionprotein

Background: Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.
Alternative Names: Cell Growth Regulator with EF Hand Domain Protein 1, Cell Growth Regulatory Gene 11 Protein, Hydrophobestin, CGREF1, CGR11

Molecular Weight: 30.9 kDa

UniProt: Q99674