CGREF1 Protein (His tag)
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- Target See all CGREF1 products
- CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CGREF1 protein is labelled with His tag.
- Purpose
- Recombinant Human CGREF1/CGR11 Protein (His Tag)
- Sequence
- Ala20-Ile301
- Characteristics
- Recombinant Human Cell Growth Regulator with EF Hand Domain Protein 1 is produced by our Mammalian expression system and the target gene encoding Ala20-Ile301 is expressed with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Cell Growth Regulator with EF-Hand Domain 1 (CGREF1) (AA 20-301) protein (His tag)
CGREF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cell Growth Regulator with EF-Hand Domain 1 (CGREF1) (AA 136-235) protein (GST tag)CGREF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cell Growth Regulator with EF-Hand Domain 1 (CGREF1) protein (Myc-DYKDDDDK Tag)CGREF1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM GaCl2, pH 7.5.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CGREF1 (Cell Growth Regulator with EF-Hand Domain 1 (CGREF1))
- Alternative Name
- CGREF1/CGR11 (CGREF1 Products)
- Synonyms
- CGR11 Protein, CGREF1 Protein, 1110004G24Rik Protein, AW045201 Protein, Cgr11 Protein, cell growth regulator with EF-hand domain 1 Protein, cell growth regulator with EF-hand domain 1 L homeolog Protein, cell growth regulator with ring finger domain 1 Protein, cell growth regulator with EF hand domain 1 Protein, CGREF1 Protein, cgref1.L Protein, cgref1 Protein, CGRRF1 Protein, Cgref1 Protein
- Background
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Background: Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.
Synonym: Cell Growth Regulator with EF Hand Domain Protein 1, Cell Growth Regulatory Gene 11 Protein, Hydrophobestin, CGREF1, CGR11
- Molecular Weight
- 30.9 kDa
- UniProt
- Q99674
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