POLD2 Protein (AA 1-469) (His tag)

Catalog No. ABIN1692161

Product Details

Target: POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit (POLD2))

Protein Type: Recombinant

Protein Characteristics: AA 1-469

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This POLD2 protein is labelled with His tag.

Purpose: Recombinant Human DNA Polymerase δ Subunit 2/POLD2 (C-6His)

Sequence: MFSEQAAQRA HTLLSPPSAN NATFARVPVA TYTNSSQPFR LGERSFSRQY AHIYATRLIQ MRPFLENRAQ QHWGSGVGVK KLCELQPEEK CCVVGTLFKA MPLQPSILRE VSEEHNLLPQ PPRSKYIHPD DELVLEDELQ RIKLKGTIDV SKLVTGTVLA VFGSVRDDGK FLVEDYCFAD LAPQKPAPPL DTDRFVLLVS GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR VILAGNLLSH STQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS DIFRYSSMED HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH VYFCGNTPSF GSKIIRGPED QTVLLVTVPD FSATQTACLV NLRSLACQPI SFSGFGAEDD DLGGLGLGPL EHHHHHH

Characteristics: Recombinant Human DNA Polymerase Delta Subunit 2/POLD2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro469) of Human POLD2 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of PBS, 10 % Glycerol, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit (POLD2))

Alternative Name: POLD2 (POLD2 Products)

Synonyms: 50kDa Protein, p50 Protein, po1D2 Protein, cdc1 Protein, si:dz150f13.3 Protein, zgc:55633 Protein, zgc:77043 Protein, DNA polymerase delta 2, accessory subunit Protein, polymerase (DNA directed), delta 2, regulatory subunit Protein, polymerase (DNA directed), delta 2, accessory subunit L homeolog Protein, POLD2 Protein, Pold2 Protein, pold2.L Protein, pold2 Protein

Sub Type: Fusionprotein

Background: DNA Polymerase Delta Subunit 2 (POLD2) belongs to the DNA polymerase delta/II small subunit family. POLD2 can form a heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. POLD2 possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. POLD2 is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA).
Alternative Names: DNA Polymerase Delta Subunit 2, DNA Polymerase Delta Subunit p50, POLD2

Molecular Weight: 52.3 kDa

UniProt: P49005

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA