PPT1 Protein (AA 28-306) (His tag)
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- Target See all PPT1 Proteins
- PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-306
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PPT1 protein is labelled with His tag.
- Purpose
- Recombinant Human Palmitoyl-Protein Thioesterase 1/PPT1 (C-6His)
- Sequence
- DPPAPLPLVI WHGMGDSCCN PLSMGAIKKM VEKKIPGIYV LSLEIGKTLM EDVENSFFLN VNSQVTTVCQ ALAKDPKLQQ GYNAMGFSQG GQFLRAVAQR CPSPPMINLI SVGGQHQGVF GLPRCPGESS HICDFIRKTL NAGAYSKVVQ ERLVQAEYWH DPIKEDVYRN HSIFLADINQ ERGINESYKK NLMALKKFVM VKFLNDSIVD PVDSEWFGFY RSGQAKETIP LQETSLYTQD RLGLKEMDNA GQLVFLATEG DHLQLSEEWF YAHIIPFLGV DHHHHHH
- Characteristics
- Recombinant Human Palmitoyl-protein thioesterase 1/PPT1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp28-Gly306) of Human PPT1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PPT1 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))
- Alternative Name
- PPT1 (PPT1 Products)
- Synonyms
- CLN1 Protein, INCL Protein, PPT Protein, 9530043G02Rik Protein, AA960502 Protein, C77813 Protein, D4Ertd184e Protein, Ppt Protein, wu:fj17f04 Protein, zgc:63969 Protein, PPT-1 Protein, ppt1 Protein, palmitoyl-protein thioesterase 1 Protein, Palmitoyl-protein thioesterase 1 Protein, palmitoyl-protein thioesterase 1 (ceroid-lipofuscinosis, neuronal 1, infantile) Protein, palmitoyl-protein thioesterase 1 L homeolog Protein, PPT1 Protein, MGYG_00692 Protein, Ppt1 Protein, ppt-1 Protein, ppt1 Protein, ppt1.L Protein
- Sub Type
- Fusionprotein
- Background
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Palmitoyl-protein thioesterase 1(PPT-1 for short), also known as Palmitoyl-protein hydrolase 1, belongs to the palmitoyl-protein thioesterase family. It is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. This enzyme removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1.
Alternative Names: Palmitoyl-protein thioesterase 1(PPT-1 for short), also known as Palmitoyl-protein hydrolase 1, belongs to the palmitoyl-protein thioesterase family. - Molecular Weight
- 32.3 kDa
- UniProt
- P50897
- Pathways
- SARS-CoV-2 Protein Interactome
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