INSL3 Protein (AA 21-131) (His tag)
INSL3
Origin: Human
Host: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target See all INSL3 Proteins
- INSL3 (Insulin-Like 3 (Leydig Cell) (INSL3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-131
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This INSL3 protein is labelled with His tag.
- Purpose
- Recombinant Human Leydig Insulin-Like 3/INSL3 (C-6His)
- Sequence
- LGPAPTPEMR EKLCGHHFVR ALVRVCGGPR WSTEARRPAT GGDRELLQWL ERRHLLHGLV ADSNLTLGPG LQPLPQTSHH HRHHRAAATN PARYCCLSGC TQQDLLTLCP YVDHHHHHH
- Characteristics
- Recombinant Human INSL3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (AA 21-131) of Human INSL3 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Insulin-Like 3 (Leydig Cell) (INSL3) protein (Myc-DYKDDDDK Tag)
INSL3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- INSL3 (Insulin-Like 3 (Leydig Cell) (INSL3))
- Alternative Name
- Leydig Insulin-Like Peptide (INSL3 Products)
- Synonyms
- INSL3 Protein, Rlf Protein, Rlnl Protein, RLF Protein, RLNL Protein, ley-I-L Protein, BLEYI-L Protein, LEY I-L Protein, Ley-I-L Protein, insulin like 3 Protein, insulin-like 3 Protein, Janus kinase 3 Protein, INSL3 Protein, Insl3 Protein, JAK3 Protein
- Background
- Insulin-like 3 is a protein that in humans is encoded by the INSL3 gene. It is a secreted protein that belongs to the insulin family. It is expressed in prenatal and postnatal Leydig cells and found as well in the corpus luteum, trophoblast, fetal membranes and breast. It may act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent.It is a ligand for LGR8 receptor.
- Molecular Weight
- 13.4 kDa
- UniProt
- P51460
- Pathways
- Hormone Activity, cAMP Metabolic Process
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