IL-1 beta Protein (AA 117-269)

Catalog No. ABIN2018039

Product Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 117-269

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50< 1.0 pg/mL, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 109 IU/mg.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Interleukin-1 beta (rhIL-1β) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Expiry Date: 6 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta (IL-1beta) (IL1B Products)

Background: Interleukin-1 beta (rhIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant human Interleukin-1 beta (rhIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rhIL-1β has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.

Molecular Weight: 17.3 kDa, observed by reducing SDS-PAGE.

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome