IL12 Protein (AA 23-215, AA 23-335)
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- Target See all IL12 Proteins
- IL12 (Interleukin 12 (IL12))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 23-215, AA 23-335
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Origin
- Mouse
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Source
- CHO Cells
- Characteristics
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ED50 < 0.15 ng/mL, measured in a cell proliferation assay using 2D6 cells.
AA 23-215 (p35) and AA 23-335 (p40), expressed with C-terminal 6xHis. - Purity
- > 95 % as analyzed by SDS-PAGE.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product IL12 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant murineInterleukin-12 (IL-12), remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, murine Interleukin-12 (IL-12), His should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- IL12 (Interleukin 12 (IL12))
- Alternative Name
- Interleukin-12 (IL-12) (IL12 Products)
- Synonyms
- CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein
- Background
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Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rbeta1 and IL-12 Rbeta2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Synonyms: NKSF, TCMF, CLMF, TSF - Molecular Weight
- 27-29 kDa (p35) and 45-50 kDa (p40), observed by reducing SDS-PAGE.
- UniProt
- P43431, P43432
- Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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