CCL15 Protein (AA 22-113, Ile24Thr-Mutant)
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- Target See all CCL15 Proteins
- CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 22-113, Ile24Thr-Mutant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Characteristics
- ED50 < 2 μg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR1, the receptor of human CCL15, corresponding to a specific activity of > 500 units/mg.
- Purity
- > 95 % by SDS-PAGE analysis.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product CCL15 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human MIP-5/CCL15 (rhMIP-5/CCL15) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhMIP-5/CCL15 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
- Alternative Name
- MIP-5 /CCL15 (CCL15 Products)
- Synonyms
- HCC-2 Protein, HMRP-2B Protein, LKN-1 Protein, LKN1 Protein, MIP-1 delta Protein, MIP-1D Protein, MIP-5 Protein, MRP-2B Protein, NCC-3 Protein, NCC3 Protein, SCYA15 Protein, SCYL3 Protein, SY15 Protein, C-C motif chemokine ligand 15 Protein, CCL15 Protein
- Background
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Macrophage Inflammatory Protein-5 (MIP-5/CCL15) is a chemokine originally identified in the human hemofiltrate, thus it is also named Hemofiltrate CC Chemokine-2 (HCC-2). MIP-5 belongs to the CCL chemokine family, and its receptors are G-protein coupled receptors CCR1 and CCR3, with CCR1 being the major one. MIP-5 is mainly expressed in heart and skeletal muscle, and CCR1 is expressed on Th1 and Th2 cells in human cord blood lymphocytes. In vivo, MIP-5 promotes the accumulation of immature myeloid cells and the expansion of metastatic foci in the lever. MIP-5 contributes to severe asthma, sarcoidosis, and atherosclerosis,however, MIP-5 can also inhibit stem cell proliferation, implicating its therapeutic potential as an alternative to high dose chemotherapy.Recombinant human MIP-5/CCL15 (rhMIP-5/CCL15) produced in E.coli is a single non-glycosylated polypeptide chain containing 92 amino acids. A fully biologically active molecule, rhMIP-5/CCL15 has a molecular mass of 10.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: HCC-2, LKN-1, MIP-1 delta - Molecular Weight
- 10.2 kDa, observed by reducing SDS-PAGE.
- UniProt
- Q16663
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