MAG Protein (AA 20-516) (His tag)

Catalog No. ABIN2115497

Product Details

Target: MAG (Myelin Associated Glycoprotein (MAG))

Protein Type: Recombinant

Protein Characteristics: AA 20-516

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This MAG protein is labelled with His tag.

Purpose: Recombinant Human Myelin-Associated Glycoprotein/MAG/Siglec-4a (C-6His)

Sequence: GHWGAWMPSS ISAFEGTCVS IPCRFDFPDE LRPAVVHGVW YFNSPYPKNY PPVVFKSRTQ VVHESFQGRS RLLGDLGLRN CTLLLSNVSP ELGGKYYFRG DLGGYNQYTF SEHSVLDIVN TPNIVVPPEV VAGTEVEVSC MVPDNCPELR PELSWLGHEG LGEPAVLGRL REDEGTWVQV SLLHFVPTRE ANGHRLGCQA SFPNTTLQFE GYASMDVKYP PVIVEMNSSV EAIEGSHVSL LCGADSNPPP LLTWMRDGTV LREAVAESLL LELEEVTPAE DGVYACLAEN AYGQDNRTVG LSVMYAPWKP TVNGTMVAVE GETVSILCST QSNPDPILTI FKEKQILSTV IYESELQLEL PAVSPEDDGE YWCVAENQYG QRATAFNLSV EFAPVLLLES HCAAARDTVQ CLCVVKSNPE PSVAFELPSR NVTVNESERE FVYSERSGLV LTSILTLRGQ AQAPPRVICT ARNLYGAKSL ELPFQGAHRL MWAKIGPVDH HHHHH

Characteristics: Recombinant Human Myelin-Associated Glycoprotein/MAG is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gly20-Pro516) of Human MAG fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG (MAG Products)

Synonyms: GMA Protein, S-MAG Protein, SIGLEC-4A Protein, SIGLEC4A Protein, siglec-4 Protein, MAG Protein, Gma Protein, siglec-4a Protein, myelin associated glycoprotein Protein, myelin-associated glycoprotein Protein, MAG Protein, mag Protein, Mag Protein

Background: Myelin-associated glycoprotein is also known as Siglec-4a, MAG and GMA,is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. MAG is a single-pass type I membrane protein and contains four Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. MAG is a adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. MAG is a functional ligand of the NOGO-66 receptor. MAG is thought be involved in myelination during nerve regeneration.
Synonyms: Myelin-Associated Glycoprotein, Siglec-4a, MAG, GMA

Molecular Weight: 55.7 kDa

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway