PDGFA Protein (AA 87-211) (His tag)

Catalog No. ABIN2115450

Product Details

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Protein Type: Recombinant

Protein Characteristics: AA 87-211

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PDGFA protein is labelled with His tag.

Purpose: Recombinant Human Platelet-Derived Growth Factor AA/PDGF-AA (N-6His)

Sequence: MNHKVHHHHH HMSIEEAVPA VCKTRTVIYE IPRSQVDPTS ANFLIWPPCV EVKRCTGCCN TSSVKCQPSR VHHRSVKVAK VEYVRKKPKL KEVQVRLEEH LECACATTSL NPDYREEDTG RPRESGKKRK RKRLKPT

Characteristics: Recombinant Human PDGFA is produced with our E. coli expression system. The target protein is expressed with sequence (Ser87-Thr211) of Human PDGFA fused with a polyhistidine tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Alternative Name: PDGFA (PDGFA Products)

Synonyms: PDGF-A Protein, PDGF1 Protein, pdgfa Protein, pdgfa-2 Protein, zgc:100777 Protein, pdgf Protein, pdgf1 Protein, pdgf-a Protein, AA409063 Protein, CD142 Protein, Cf-3 Protein, Cf3 Protein, TF Protein, PDGFACP Protein, wu:fc32c12 Protein, zgc:153249 Protein, platelet derived growth factor subunit A Protein, platelet-derived growth factor alpha polypeptide a Protein, coagulation factor III Protein, platelet derived growth factor, alpha Protein, platelet derived growth factor subunit A S homeolog Protein, platelet-derived growth factor alpha polypeptide b Protein, PDGFA Protein, pdgfaa Protein, pdgfa Protein, F3 Protein, Pdgfa Protein, pdgfa.S Protein, pdgfab Protein

Background: Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing, Signaling is modulated by the formation of heterodimers with PDGFB.
Synonyms: Platelet-derived growth factor subunit A,PDGF subunit A,PDGF-1,Platelet-derived growth factor A chain,Platelet-derived growth factor alpha polypeptide, PDGFA,PDGF1

Molecular Weight: 15.9 kDa

UniProt: P04085

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling