IL12A Protein (AA 23-219) (Fc Tag)

Catalog No. ABIN2181329

Product Details

Target: IL12A (Interleukin 12 alpha (IL12A))

Protein Type: Recombinant

Protein Characteristics: AA 23-219

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12A protein is labelled with Fc Tag.

Sequence: AA 23-219

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >90 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Target Details

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: IL-12A (IL12A Products)

Synonyms: CLMF Protein, IL-12A Protein, NFSK Protein, NKSF1 Protein, P35 Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p35 Protein, Il-12 p35 Protein, IL12p40 Protein, IL12 Protein, IL-12 p35 Protein, interleukin 12A Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12A Protein, IL12B Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Background: Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit alpha (IL12A) also known as NKSF1, CLMF1 and P35, IL12A shows significant sequence similarity to IL-6, G-CSF, and exerts biological activities only when the IL12B is co-expressed. IL12B deficient mice are resistant to the induction of experimental chronic inflammatory diseases whereas IL12A knock-out mice develop more severe forms, suggesting opposite functions of the two subunits in the outcome of chronic inflammatory diseases.

Molecular Weight: 49.2 kDa

UniProt: P29459

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation