ICAM1 Protein (AA 28-485, C-Term) (Fc Tag)

Catalog No. ABIN2666825

Product Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 28-485, C-Term

Origin: Mouse

Source: CHO Cells

Purification tag / Conjugate: This ICAM1 protein is labelled with Fc Tag.

Application: ELISA, Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 1.0 EU per μg of protein as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Immobilized mouse ICAM-1-Fc chimera is able to bind retinoic acid (2 μM, 72 hr) activated HL60 cells (5 x 104 /well) in a dose dependent manner. Over 80% of the cells were able to bind mouse ICAM-1 at a dose greater than 12.5 μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Concentration: 100 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM-1 (ICAM1 Products)

Synonyms: BB2 Protein, CD54 Protein, P3.58 Protein, Icam-1 Protein, Ly-47 Protein, MALA-2 Protein, ICAM Protein, ICAM-1 Protein, ICAM1 Protein, intercellular adhesion molecule 1 Protein, intercellular adhesion molecule-1 Protein, ICAM1 Protein, Icam1 Protein, ICAM-1 Protein

Target Type: Viral Protein

Background: ICAM-1, intercellular adhesion molecule 1, is a member of the immunoglobulin superfamily. Structurely, ICAM-1 is characterized by heavy glycosylation with an extracellular domain composed of multiple loops created by disulfide bridges within the protein. The dominant secondary structure of the protein is the beta sheet, leading investigators to hypothesize the presence of dimerization domains within ICAM-1. This protein is constitutively expressed at low levels on the cell surface of leukocytes, endothelial cells, macrophages, and lymphocytes. Upon cytokine activation, by cytokines such as IL-1 and TNFα, the concentration of ICAM-1 on the cell surface greatly increases. The ligand of ICAM-1 is LFA-1 (leukocyte function associated antigen 1), which is found on all T cells. LFA-1/ICAM-1 interaction has been shown to be important for T cell-T cell interactions, which leads to further T cell differentiation. ICAM-1 was found to play a role in infectious diseases, as it was identified as the binding target for entry of the major group of human rhinoviruses into various cell types and is also known for its affinity for Plasmodium falciparum-infected erythrocytes. These findings indicate that ICAM-1 may participate in the pathogenesis of several infectious agents

Molecular Weight: The 696 amino acid recombinant protein, with a C-terminal human IgG1 Fc tag, has a predicted molecular mass of approximately 77 kDa. The protein migrates near 130 kDa by SDS-PAGE in DTT-reducing conditions and near 250 kDa in non-reducing conditions. The

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling