ABCB1 Protein (DYKDDDDK Tag)
Quick Overview for ABCB1 Protein (DYKDDDDK Tag) (ABIN2713531)
Target
See all ABCB1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ABCB1 protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human CD243 / MDR1 (C-term DDK tag) protein expressed in sf9 cells.
- Produced with end-sequenced ORF clone
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ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 1-1280) protein (His tag)
custom-made ABCB1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 620-709) protein (GST tag)ABCB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 491-694) protein (His-IF2DI Tag)ABCB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) protein (His tag)ABCB1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))
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Alternative Name
- Cd243,mdr1
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Background
- The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
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Molecular Weight
- 31.3 kDa
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NCBI Accession
- NP_000918
Target
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