Angiopoietin 1 Protein (ANGPT1) (Transcript Variant 1) (His tag)
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- Target See all Angiopoietin 1 (ANGPT1) Proteins
- Angiopoietin 1 (ANGPT1)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HeLa Cells
- Purification tag / Conjugate
- This Angiopoietin 1 protein is labelled with His tag.
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Angiopoietin-1 (transcript variant 1) protein expressed in HeLa cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
- Top Product
- Discover our top product ANGPT1 Protein
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Angiopoietin 1 (ANGPT1) (AA 1-498) protein (GST tag)
ANGPT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Angiopoietin 1 (ANGPT1) (AA 221-320) protein (GST tag)ANGPT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Angiopoietin 1 (ANGPT1) protein (Fc Tag)ANGPT1 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Angiopoietin 1 (ANGPT1)
- Alternative Name
- Angiopoietin-1 (ANGPT1 Products)
- Synonyms
- AGP1 Protein, AGPT Protein, ANG1 Protein, 1110046O21Rik Protein, Ang-1 Protein, Ang1 Protein, Agpt Protein, Agpt1 Protein, ang1 Protein, wu:fc76g06 Protein, ANG-1 Protein, angpt1 Protein, MGC80788 Protein, angiopoietin 1 Protein, angiopoietin 1 S homeolog Protein, ANGPT1 Protein, Angpt1 Protein, angpt1 Protein, angpt1.S Protein
- Background
- This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
- Molecular Weight
- 57.3
- NCBI Accession
- NP_001137
- Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process
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