HLA-E Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722835

Product Details

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HLA-E protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human HLA class I alpha E / HLA-E protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: Hla Class I alpha E,hla-E (HLA-E Products)

Synonyms: EA1.2 Protein, EA2.1 Protein, HLA-6.2 Protein, MHC Protein, QA1 Protein, major histocompatibility complex, class I, E Protein, HLA-E Protein, PATR-E Protein

Background: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

Molecular Weight: 37.8 kDa

NCBI Accession: NP_005507

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process