PHLPP1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728848

Product Details

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PHLPP1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human PHLPP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Teng, Sun, An, Hu, Liu, Ma, Han, Shi: "Role of PHLPP1 in inflammation response: Its loss contributes to gliomas development and progression." in: International immunopharmacology, Vol. 34, pp. 229-34, (2016) (PubMed).

Lin, Lin, Wu, Ballard, Lee, Gloor, Vigers, Morales, Friedman, Skelton, Brandhuber: "An ATP-site on-off switch that restricts phosphatase accessibility of Akt." in: Science signaling, Vol. 5, Issue 223, pp. ra37, (2012) (PubMed).

Target Details

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Alternative Name: Phlpp1 (PHLPP1 Products)

Synonyms: PHLPP Protein, PLEKHE1 Protein, SCOP Protein, AI836256 Protein, Phlpp Protein, Plekhe1 Protein, mKIAA0606 Protein, Scop Protein, PH domain and leucine rich repeat protein phosphatase 1 Protein, PHLPP1 Protein, phlpp1 Protein, Phlpp1 Protein

Background: This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Molecular Weight: 184.5 kDa

NCBI Accession: NP_919431

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway