PKC zeta Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all PKC zeta (PRKCZ) Proteins
- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PKC zeta protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PRKCZ (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PRKCZ Protein
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Protein Kinase C, zeta (PRKCZ) (AA 6-584) (Active) protein (His-GST)
PRKCZ Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Protein Kinase C, zeta (PRKCZ) (AA 454-592) protein (His-IF2DI Tag)PRKCZ Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (GST tag)PRKCZ Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase C, zeta (PRKCZ) (AA 165-255) protein (GST tag)PRKCZ Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (Strep Tag)Crystallography grade PRKCZ Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (Strep Tag)Crystallography grade PRKCZ Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
- Alternative Name
- Prkcz (PRKCZ Products)
- Synonyms
- TPKC Protein, PKCzeta Protein, prkcz-A Protein, pkc-zeta Protein, PRKCZ Protein, pkc2 Protein, PKC-ZETA Protein, PKC2 Protein, AI098070 Protein, C80388 Protein, Pkcz Protein, R74924 Protein, aPKCzeta Protein, zetaPKC Protein, 14-3-3-zetaisoform Protein, r14-3-3 Protein, pkcz Protein, si:ch211-150o23.4 Protein, protein kinase C zeta L homeolog Protein, protein kinase C zeta Protein, protein kinase C, zeta Protein, prkcz.L Protein, PRKCZ Protein, prkcz Protein, Prkcz Protein
- Background
- Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
- Molecular Weight
- 67.5 kDa
- NCBI Accession
- NP_002735
- Pathways
- NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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