PPM1D Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729806

Product Details

Target: PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PPM1D protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Protein phosphatase 1D / PPM1D protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))

Alternative Name: Protein Phosphatase 1d,ppm1d (PPM1D Products)

Synonyms: PP2C-DELTA Protein, WIP1 Protein, AV338790 Protein, Wip1 Protein, zgc:103518 Protein, ppm1d Protein, wu:fj44c08 Protein, zgc:55836 Protein, zgc:77316 Protein, protein phosphatase, Mg2+/Mn2+ dependent 1D Protein, protein phosphatase 1D magnesium-dependent, delta isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1D Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Db Protein, protein phosphatase, Mg2+/Mn2+ dependent 1D L homeolog Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1Da Protein, PPM1D Protein, Ppm1d Protein, ppm1db Protein, ppm1d.L Protein, ppm1da Protein

Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Molecular Weight: 66.5 kDa

NCBI Accession: NP_003611

Pathways: p53 Signaling, Cell Division Cycle