Flagellin Protein (FliC) (AA 1-495) (His tag)
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- Target See all Flagellin (FliC) Proteins
- Flagellin (FliC)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-495
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Origin
- Human, Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Flagellin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Specificity
- Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).
- Cross-Reactivity
- Human, Mouse (Murine)
- Characteristics
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product FliC Protein
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Flagellin (FliC) protein
FliC Origin: Borrelia burgdorferi Host: Escherichia coli (E. coli) Recombinant ELISA, WB
Flagellin (FliC) (AA 2-498) protein (His tag)FliC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5μg/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25μg/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 100 μL sterile water.
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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- Target
- Flagellin (FliC)
- Alternative Name
- Flagellin (FliC Products)
- Synonyms
- hag Protein, flaG Protein, flagellin Protein, flagellin (flic) Protein, flagellin E Protein, flagellin protein flic Protein, fliC Protein, FliC Protein, fliC+F2048 Protein, CJJ81176_1339 Protein, C8J_1256 Protein
- Background
- Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
- UniProt
- P06179
- Pathways
- Inflammasome
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