ZC3H7A Protein (AA 1-971) (Strep Tag)

Catalog No. ABIN3116340

Product Details

Target: ZC3H7A (Zinc Finger CCCH-Type Containing 7A (ZC3H7A))

Protein Type: Recombinant

Protein Characteristics: AA 1-971

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZC3H7A protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSNVSEERRK RQQNIKEGLQ FIQSPLSYPG TQEQYAVYLR ALVRNLFNEG NDVYREHDWN NSISQYTEAL NIADYAKSEE ILIPKEIIEK LYINRIACYS NMGFHDKVLE DCNIVLSLNA SNCKALYRKS KALSDLGRYK KAYDAVAKCS LAVPQDEHVI KLTQELAQKL GFKIRKAYVR AELSLKSVPG DGATKALNHS VEDIEPDLLT PRQEAVPVVS LPAPSFSHEV GSELASVPVM PLTSILPLQV EESALPSAVL ANGGKMPFTM PEAFLDDGDM VLGDELDDLL DSAPETNETV MPSALVRGPL QTASVSPSMP FSASLLGTLP IGARYAPPPS FSEFYPPLTS SLEDFCSSLN SFSMSESKRD LSTSTSREGT PLNNSNSSLL LMNGPGSLFA SENFLGISSQ PRNDFGNFFG SAVTKPSSSV TPRHPLEGTH ELRQACQICF VKSGPKLMDF TYHANIDHKC KKDILIGRIK NVEDKSWKKI RPRPTKTNYE GPYYICKDVA AEEECRYSGH CTFAYCQEEI DVWTLERKGA FSREAFFGGN GKINLTVFKL LQEHLGEFIF LCEKCFDHKP RMISKRNKDN STACSHPVTK HEFEDNKCLV HILRETTVKY SKIRSFHGQC QLDLCRHEVR YGCLREDECF YAHSLVELKV WIMQNETGIS HDAIAQESKR YWQNLEANVP GAQVLGNQIM PGFLNMKIKF VCAQCLRNGQ VIEPDKNRKY CSAKARHSWT KDRRAMRVMS IERKKWMNIR PLPTKKQMPL QFDLCNHIAS GKKCQYVGNC SFAHSPEERE VWTYMKENGI QDMEQFYELW LKSQKNEKSE DIASQSNKEN GKQIHMPTDY AEVTVDFHCW MCGKNCNSEK QWQGHISSEK HKEKVFHTED DQYCWQHRFP TGYFSICDRY MNGTCPEGNS CKFAHGNAEL HEWEERRDAL KMKLNKARKD HLIGPNDNDF GKYSFLFKDL N
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZC3H7A (Zinc Finger CCCH-Type Containing 7A (ZC3H7A))

Alternative Name: ZC3H7A (ZC3H7A Products)

Synonyms: zc3h7 Protein, fa12e01 Protein, fc07f05 Protein, fc38e12 Protein, fc55c11 Protein, zgc:55684 Protein, wu:fa12e01 Protein, wu:fc07f05 Protein, wu:fc38e12 Protein, wu:fc55c11 Protein, zc3hdc7 Protein, MGC81244 Protein, hspc055 Protein, A430104C18Rik Protein, AI447294 Protein, AW556219 Protein, HSPC055 Protein, Zc3h7 Protein, Zc3hdc7 Protein, ZC3H7 Protein, ZC3HDC7 Protein, zinc finger CCCH-type containing 7A Protein, zinc finger CCCH-type containing 7A L homeolog Protein, zinc finger CCCH type containing 7 A Protein, zc3h7a Protein, ZC3H7A Protein, zc3h7a.L Protein, Zc3h7a Protein

Background: Zinc finger CCCH domain-containing protein 7A,FUNCTION: May be a specific regulator of miRNA biogenesis. Binds to microRNAs MIR7-1, MIR16-2 and MIR29A hairpins recognizing the 3'-ATA(A/T)-5' motif in the apical loop. {ECO:0000269|PubMed:28431233}.

Molecular Weight: 110.5 kDa

UniProt: Q8IWR0

Pathways: SARS-CoV-2 Protein Interactome