NLRP9 Protein (AA 1-991) (Strep Tag)

Catalog No. ABIN3093985

Recombinant NLRP9 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for NLRP9 Protein (AA 1-991) (Strep Tag) (ABIN3093985)

Target: NLRP9 (NLR Family, Pyrin Domain Containing 9 (NLRP9))

Protein Type: Recombinant

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-991

Purification tag / Conjugate: This NLRP9 protein is labelled with Strep Tag.

Sequence: MAESFFSDFG LLWYLKELRK EEFWKFKELL KQPLEKFELK PIPWAELKKA SKEDVAKLLD KHYPGKQAWE VTLNLFLQIN RKDLWTKAQE EMRNKLNPYR KHMKETFQLI WEKETCLHVP EHFYKETMKN EYKELNDAYT AAARRHTVVL EGPDGIGKTT LLRKVMLDWA EGNLWKDRFT FVFFLNVCEM NGIAETSLLE LLSRDWPESS EKIEDIFSQP ERILFIMDGF EQLKFNLQLK ADLSDDWRQR QPMPIILSSL LQKKMLPESS LLIALGKLAM QKHYFMLRHP KLIKLLGFSE SEKKSYFSYF FGEKSKALKV FNFVRDNGPL FILCHNPFTC WLVCTCVKQR LERGEDLEIN SQNTTYLYAS FLTTVFKAGS QSFPPKVNRA RLKSLCALAA EGIWTYTFVF SHGDLRRNGL SESEGVMWVG MRLLQRRGDC FAFMHLCIQE FCAAMFYLLK RPKDDPNPAI GSITQLVRAS VVQPQTLLTQ VGIFMFGIST EEIVSMLETS FGFPLSKDLK QEITQCLESL SQCEADREAI AFQELFIGLF ETQEKEFVTK VMNFFEEVFI YIGNIEHLVI ASFCLKHCQH LTTLRMCVEN IFPDDSGCIS DYNEKLVYWR ELCSMFITNK NFQILDMENT SLDDPSLAIL CKALAQPVCK LRKLIFTSVY FGHDSELFKA VLHNPHLKLL SLYGTSLSQS DIRHLCETLK HPMCKIEELI LGKCDISSEV CEDIASVLAC NSKLKHLSLV ENPLRDEGMT LLCEALKHSH CALERLMLMY CCLTSVSCDS ISEVLLCSKS LSLLDLGSNA LEDNGVASLC AALKHPGCSI RELWLMGCFL TSDSCKDIAA VLICNGKLKT LKLGHNEIGD TGVRQLCAAL QHPHCKLECL GLQTCPITRA CCDDIAAALI ACKTLRSLNL DWIALDADAV VVLCEALSHP DCALQMLGLH KSGFDEETQK ILMSVEEKIP HLTISHGPWI DEEYKIRGVL L
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NLRP9 (NLR Family, Pyrin Domain Containing 9 (NLRP9))

Alternative Name: NLRP9

Background: NACHT, LRR and PYD domains-containing protein 9 (Nucleotide-binding oligomerization domain protein 6) (PYRIN and NACHT-containing protein 12),FUNCTION: As the sensor component of the NLRP9 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens, including rotavirus, initiates the formation of the inflammasome polymeric complex, made of NLRP9, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and release in the extracellular milieu. The active cytokines stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. NLRP9 inflammasome activation may be initiated by DHX9 interaction with viral double-stranded RNA (dsRNA), preferentially to short dsRNA segments. {ECO:0000269|PubMed:28636595}.

Molecular Weight: 113.3 kDa

UniProt: Q7RTR0

Pathways: Inflammasome