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HAS1 Protein (AA 1-578) (Strep Tag)

HAS1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). SDS, WB, ELISA
Catalog No. ABIN3117112
  • Target See all HAS1 Proteins
    HAS1 (Hyaluronan Synthase 1 (HAS1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-578
    Origin
    • 3
    • 2
    • 1
    Human
    Source
    • 4
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This HAS1 protein is labelled with Strep Tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA
    Sequence
    MRQQDAPKPT PAACRCSGLA RRVLTIAFAL LILGLMTWAY AAGVPLASDR YGLLAFGLYG AFLSAHLVAQ SLFAYLEHRR VAAAARGPLD AATARSVALT ISAYQEDPAY LRQCLASARA LLYPRARLRV LMVVDGNRAE DLYMVDMFRE VFADEDPATY VWDGNYHQPW EPAAAGAVGA GAYREVEAED PGRLAVEALV RTRRCVCVAQ RWGGKREVMY TAFKALGDSV DYVQVCDSDT RLDPMALLEL VRVLDEDPRV GAVGGDVRIL NPLDSWVSFL SSLRYWVAFN VERACQSYFH CVSCISGPLG LYRNNLLQQF LEAWYNQKFL GTHCTFGDDR HLTNRMLSMG YATKYTSRSR CYSETPSSFL RWLSQQTRWS KSYFREWLYN ALWWHRHHAW MTYEAVVSGL FPFFVAATVL RLFYAGRPWA LLWVLLCVQG VALAKAAFAA WLRGCLRMVL LSLYAPLYMC GLLPAKFLAL VTMNQSGWGT SGRRKLAANY VPLLPLALWA LLLLGGLVRS VAHEARADWS GPSRAAEAYH LAAGAGAYVG YWVAMLTLYW VGVRRLCRRR TGGYRVQV
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product HAS1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    HAS1 (Hyaluronan Synthase 1 (HAS1))
    Alternative Name
    HAS1 (HAS1 Products)
    Synonyms
    dg42 Protein, Xhas1 Protein, HAS Protein, shas1 Protein, hyaluronan synthase 1 Protein, hyaluronan synthase 1 S homeolog Protein, has1 Protein, HAS1 Protein, Has1 Protein, has1.S Protein
    Background
    Hyaluronan synthase 1 (EC 2.4.1.212) (Hyaluronate synthase 1) (Hyaluronic acid synthase 1) (HA synthase 1) (HuHAS1),FUNCTION: Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate (By similarity). {ECO:0000250}.
    Molecular Weight
    64.8 kDa
    UniProt
    Q92839
    Pathways
    Glycosaminoglycan Metabolic Process
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