NUTF2 Protein (AA 1-127) (Strep Tag)
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- Target See all NUTF2 Proteins
- NUTF2 (Nuclear Transport Factor 2 (NUTF2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-127
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This NUTF2 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product NUTF2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- NUTF2 (Nuclear Transport Factor 2 (NUTF2))
- Alternative Name
- Nutf2 (NUTF2 Products)
- Synonyms
- NTF2 Protein, PP15 Protein, DDBDRAFT_0167060 Protein, DDBDRAFT_0233957 Protein, DDB_0167060 Protein, DDB_0233957 Protein, F13K9.26 Protein, F13K9_26 Protein, nuclear transport factor 2B Protein, 2700067I02Rik Protein, AI115459 Protein, AI413596 Protein, AW546000 Protein, Ntf2 Protein, Ntf3 Protein, NTF-2 Protein, ntf2 Protein, nutf2 Protein, pp15 Protein, fb40a01 Protein, wu:fb40a01 Protein, zgc:101555 Protein, nuclear transport factor 2 Protein, nuclear transport factor 2, putative Protein, nuclear transport factor 2B Protein, nuclear transport factor Nxt2 Protein, nuclear transport factor 2 L homeolog Protein, NUTF2 Protein, TP02_0050 Protein, TA13190 Protein, CC1G_11512 Protein, Bcenmc03_3881 Protein, nutf2 Protein, Nutf2 Protein, NTF2B Protein, nxt2 Protein, nutf2.L Protein
- Background
- Nuclear transport factor 2 (NTF-2),FUNCTION: Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import. {ECO:0000250|UniProtKB:P61970}.
- Molecular Weight
- 14.5 kDa
- UniProt
- P61971
- Pathways
- Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome
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