SCYL1 Protein (AA 1-806) (Strep Tag)
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- Target See all SCYL1 Proteins
- SCYL1 (SCY1-Like 1 (SCYL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-806
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This SCYL1 protein is labelled with Strep Tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Sequence
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MWFFARDPVR DFPFELSPEP PEGGPPGPWI LHRGRKKATG SAVSIFVYDV KPGAEEQTQV AKAAFKRLKT LRHPNILAYI DGLETEKCLH IVTEAVTPLG TYLKARAEAG GLKEQELSWG LHQIVKALSF LVNDCNLIHN NVCMAAVFVD RAGEWKLGGL DYMYSAQGNG GGPPSKGIPE LEQYDPPELA DSSSRAVREK WSADMWRLGC LIWEVFNGSL PRAAALRNPG KIPKSLVTHY CELVGANPKV RPNPARFLQN CRAPGGFMSN RFVETNLFLE EIQIKEPAEK QKFFQELSKS LDSFPEDFCR HKVLPQLLTA FEFGNAGAVV LTPLFKVGKS LRAEEYQEKI IPVVVKMFSS TDRAMRIRLL QQMEQFIQYL DEPTVNTQIF PHVTHGFLDT NPAIREQTVK SMLLLAPKLN EANLNVELMK HFARLQAKDD QGPIRCNTTV CLGKIGSYLS ASTRHRVLTS AFSRATKDPF APSRVAGVLG FAATHNLYSM DDCAHKILPV LCGLTVDPEK SVRDQAFKTI RSFLSKLESV SEDPTQLAEV EKDVHAASSP GTGGAAASWA GWAVTGVSSL TSKLIRAHPT PVPSDTTVPQ RPVPEGNPAP APALAQAIPA TSGHWETQED KDTAEDSATA DRWDDEDWGS LEQEAESVLA QQDDWSAKGQ GSRAGQINHP DHKSLESHWS SWEVEGSWDQ GWQEPSSVEP PPEGTRLASE YNWGGAEPSD KGDPFAALSV RPSAQPRPDP DSWGEDNWEG LEAESRQVKA ELARKKREER RREMEAKRAE KKTTKGPMKL GARKLD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product SCYL1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SCYL1 (SCY1-Like 1 (SCYL1))
- Alternative Name
- Scyl1 (SCYL1 Products)
- Synonyms
- DDBDRAFT_0189357 Protein, DDBDRAFT_0220502 Protein, DDB_0189357 Protein, DDB_0220502 Protein, GKLP Protein, NKTL Protein, NTKL Protein, P105 Protein, TAPK Protein, TEIF Protein, TRAP Protein, 2810011O19Rik Protein, C85140 Protein, Ntkl Protein, mdf Protein, mfd Protein, p105 Protein, N-terminal kinase-like protein Protein, SCY1 like pseudokinase 1 Protein, SCY1-like, kinase-like 1 L homeolog Protein, SCY1-like 1 (S. cerevisiae) Protein, scy1 Protein, PITG_12105 Protein, SCYL1 Protein, LOC592987 Protein, scyl1.L Protein, Scyl1 Protein
- Background
- N-terminal kinase-like protein (105 kDa kinase-like protein) (Mitosis-associated kinase-like protein NTKL) (SCY1-like protein 1),FUNCTION: Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology. Has no detectable kinase activity in vitro. {ECO:0000250|UniProtKB:Q96KG9}.
- Molecular Weight
- 89.2 kDa
- UniProt
- Q9EQC5
- Pathways
- p53 Signaling
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