AATF Protein (AA 2-526) (His tag)
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- Target See all AATF Proteins
- AATF (Apoptosis Antagonizing Transcription Factor (AATF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-526
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This AATF protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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AAPQPLALQL EQLLNPRPRE ADPEADPEEA TRARVIDRFD EGEEEKDDLA VSSIRKLAPV SLLDTDKRYS GKTTSRKAWK EDHWEQALPS SSDNEASDEG GSEDGDSEGL GLEEISEDVD EDLEDNKISD EGGSEDGDSE GLGLEEFSED VEEDLEGEDE EDREEDRNSE DDGVVAAFSS VKVSEEVEKG RAVKNQIALW DQLLEGRIKL QKALLTTNQL PQPDVFPVFK DKGGPEFASA LKNSHKALKA LLRSLVDLQE ELLFQYPDTR HIVNGAKPNT ESEEISSEDD ELVGEKKKQR KAPPKRKLEM EDYPSFMAKR FADFTIYRNH TLQKWHDKTK LASGKLGKGF GAFERSILTQ IDHIMMDKER LLRRTQTKRS AYRVLGKPEP VPEPVAETLP GEPETLPQGP ANAHLRDLDE EIFDDDDFYH QLLRELIERK TSSLDPNDQV AMGRQWLAIQ KLRSKIRKKV DRKASKGRKL RFHVLSKLLS FMAPIDHTAM SDDARTELFR SLFGQLNPPD ADRGK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Aatf Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product AATF Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- AATF (Apoptosis Antagonizing Transcription Factor (AATF))
- Alternative Name
- Aatf (AATF Products)
- Synonyms
- AATF Protein, ded Protein, che1 Protein, che-1 Protein, cb109 Protein, sb:cb109 Protein, wu:fc13d05 Protein, zgc:162071 Protein, CHE-1 Protein, CHE1 Protein, DED Protein, 4933415H02Rik Protein, 5830465M17Rik Protein, Che-1 Protein, Trb Protein, Ded Protein, apoptosis antagonizing transcription factor Protein, Apoptosis antagonizing transcription factor Protein, apoptosis antagonizing transcription factor S homeolog Protein, AATF Protein, aatf Protein, BFR2 Protein, NAEGRDRAFT_56916 Protein, Aatf Protein, aatf.S Protein
- Background
- May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression (By similarity). {ECO:0000250}.
- Molecular Weight
- 60.3 kDa Including tag.
- UniProt
- Q9JKX4
- Pathways
- Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome
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