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Calsequestrin Protein (AA 35-405) (His tag)

Crystallography grade CASQ1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3131412
$9,343.16
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Delivery in 30 to 35 Business Days
  • Target See all Calsequestrin (CASQ1) Proteins
    Calsequestrin (CASQ1) (Calsequestrin 1 (CASQ1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 35-405
    Origin
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 4
    • 4
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Calsequestrin protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    EDGLDFPEYD GVDRVINVNA KNYKNVFKKY EVLALLYHEP PEDDKASQRQ FEMEELILEL AAQVLEDKGV GFGLVDSEKD AAVAKKLGLT EEDSVYVFKG DEVIEYDGEF SADTLVEFLL DVLEDPVELI EGERELQAFE NIEDEIKLIG YFKSKDSEHY KAYEDAAEEF HPYIPFFATF DSKVAKKLTL KLNEIDFYEA FMEEPMTIPD KPNSEEEIVS FVEEHRRSTL RKLKPESMYE TWEDDLDGIH IVAFAEEADP DGYEFLETLK AVAQDNTENP DLSIIWIDPD DFPLLVPYWE KTFDIDLSAP QIGVVNVTDA DSIWMEMDNE EDLPSADELE DWLEDVLEGE INTEDDDDDD DDDDDDDDDD D
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Casq1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Calsequestrin (CASQ1) (Calsequestrin 1 (CASQ1))
    Alternative Name
    Casq1 (CASQ1 Products)
    Synonyms
    CASQ Protein, PDIB1 Protein, casq1 Protein, CSQ Protein, CSQ-1 Protein, CSQ1 Protein, sCSQ Protein, zgc:154027 Protein, asph Protein, casq Protein, casq1.L Protein, pdib1 Protein, calsequestrin 1 Protein, Calsequestrin-1 Protein, calsequestrin 1b Protein, calsequestrin 1 S homeolog Protein, CASQ1 Protein, casq1 Protein, Casq1 Protein, casq1b Protein, casq1.S Protein
    Background
    Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (By similarity). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1, this plays an important role in triggering muscle contraction. {ECO:0000250|UniProtKB:P31415, ECO:0000269|PubMed:17627988, ECO:0000269|PubMed:22049211, ECO:0000269|PubMed:7945294}.
    Molecular Weight
    43.7 kDa Including tag.
    UniProt
    O09165
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