MSH3 Protein (AA 1-1091) (Strep Tag)

Catalog No. ABIN3132954

Product Details

Target: MSH3 (MutS Homolog 3 (MSH3))

Protein Type: Recombinant

Protein Characteristics: AA 1-1091

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MSH3 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MPRGKSASGG STAAGPGPGR QTVLSRFFRS AGSLRSSASS TEPAEKVTEG DSRKRSLGNG GPTKKKARKV PEKEEENISV ASHHPEAKKC LRPRIVLKSL EKLKEFCCDS ALPQNRVQTE ALRERLEVLP RCTDFEDITL QRAKNAVLSE DSKSQANQKD SQFGPCPEVF QKTSDCKPFN KRSKSVYTPL ELQYLDMKQQ HKDAVLCVEC GYKYRFFGED AEIAARELNI YCHLDHNFMT ASIPTHRLFV HVRRLVAKGY KVGVVKQTET AALKAIGDNK SSVFSRKLTA LYTKSTLIGE DVNPLIRLDD SVNIDEVMTD TSTNYLLCIY EEKENIKDKK KGNLSVGVVG VQPATGEVVF DCFQDSASRL ELETRISSLQ PVELLLPSDL SEPTEMLIQR ATNVSVRDDR IRVERMNNTY FEYSHAFQTV TEFYAREIVD SQGSQSLSGV INLEKPVICA LAAVIRYLKE FNLEKMLSKP ESFKQLSSGM EFMRINGTTL RNLEMVQNQT DMKTKGSLLW VLDHTKTSFG RRKLKNWVTQ PLLKLREINA RLDAVSDVLH SESSVFEQIE NLLRKLPDVE RGLCSIYHKK CSTQEFFLIV KSLCQLKSEL QALMPAVNSH VQSDLLRALI VELLSPVEHY LKVLNGPAAK VGDKTELFKD LSDFPLIKKR KNEIQEVIHS IQMRLQEFRK ILKLPSLQYV TVSGQEFMIE IKNSAVSCIP ADWVKVGSTK AVSRFHPPFI VESYRRLNQL REQLVLDCNA EWLGFLENFG EHYHTLCKAV DHLATVDCIF SLAKVAKQGN YCRPTLQEEK KIIIKNGRHP MIDVLLGEQD QFVPNSTSLS DSERVMIITG PNMGGKSSYI KQVTLVTIMA QIGSYVPAEE ATIGIVDGIF TRMGAADNIY KGRSTFMEQL TDTAEIIRRA SPQSLVILDE LGRGTSTHDG IAIAYATLEY FIRDVKSLTL FVTHYPPVCE LEKCYPEQVG NYHMGFLVNE DESKQDSGDM EQMPDSVTFL YQITRGIAAR SYGLNVAKLA DVPREVLQKA AHKSKELEGL VSLRRKRLEC FTDLWMTHSV KDLHTWADKL EMEEIQTSLP H
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MSH3 (MutS Homolog 3 (MSH3))

Alternative Name: Msh3 (MSH3 Products)

Synonyms: ATMSH3 Protein, M7J2.90 Protein, M7J2_90 Protein, homolog of DNA mismatch repair protein MSH3 Protein, DUP Protein, MRP1 Protein, D13Em1 Protein, Rep-3 Protein, Rep3 Protein, mutS homolog 3 Protein, DNA mismatch repair protein MSH3 Protein, homolog of DNA mismatch repair protein MSH3 Protein, mismatch repair protein Protein, MSH3 Protein, Msh3 Protein

Background: DNA mismatch repair protein Msh3 (Protein repair-1) (REP-1) (Protein repair-3) (REP-3),FUNCTION: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity). {ECO:0000250}.

Molecular Weight: 123.1 kDa

UniProt: P13705

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response