MASP1 Protein (AA 25-704) (His tag)
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- Target See all MASP1 Proteins
- MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-704
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This MASP1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequence
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HTVELNEMFG QIQSPGYPDS YPSDSEVTWN ITVPEGFRIK LYFMHFNLES SYLCEYDYVK VETEDQVLAT FCGRETTDTE QTPGQEVVLS PGTFMSVTFR SDFSNEERFT GFDAHYMAVD VDECKEREDE ELSCDHYCHN YIGGYYCSCR FGYILHTDNR TCRVECSGNL FTQRTGTITS PDYPNPYPKS SECSYTIDLE EGFMVSLQFE DIFDIEDHPE VPCPYDYIKI KAGSKVWGPF CGEKSPEPIS TQTHSVQILF RSDNSGENRG WRLSYRAAGN ECPKLQPPVY GKIEPSQAVY SFKDQVLVSC DTGYKVLKDN EVMDTFQIEC LKDGAWSNKI PTCKIVDCGA PAGLKHGLVT FSTRNNLTTY KSEIRYSCQQ PYYKMLHNTT GVYTCSAHGT WTNEVLKRSL PTCLPVCGVP KFSRKQISRI FNGRPAQKGT MPWIAMLSHL NGQPFCGGSL LGSNWVLTAA HCLHQSLDPE EPTLHSSYLL SPSDFKIIMG KHWRRRSDED EQHLHVKRTT LHPLYNPSTF ENDLGLVELS ESPRLNDFVM PVCLPEQPST EGTMVIVSGW GKQFLQRFPE NLMEIEIPIV NSDTCQEAYT PLKKKVTKDM ICAGEKEGGK DACAGDSGGP MVTKDAERDQ WYLVGVVSWG EDCGKKDRYG VYSYIYPNKD WIQRITGVRN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Masp1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product MASP1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))
- Alternative Name
- Masp1 (MASP1 Products)
- Synonyms
- AW048060 Protein, CCPII Protein, Crarf Protein, Masp1/3 Protein, 3MC1 Protein, CRARF Protein, CRARF1 Protein, MAP1 Protein, MASP Protein, MASP3 Protein, MAp44 Protein, PRSS5 Protein, RaRF Protein, mannan-binding lectin serine peptidase 1 Protein, mannan binding lectin serine peptidase 1 Protein, Masp1 Protein, MASP1 Protein
- Background
- Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. {ECO:0000269|PubMed:18424734}.
- Molecular Weight
- 78.1 kDa Including tag.
- UniProt
- P98064
- Pathways
- Complement System
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