Cyclin B3 Protein (CCNB3) (AA 1-1396) (Strep Tag)

Catalog No. ABIN3136098

Product Details

Target: Cyclin B3 (CCNB3)

Protein Type: Recombinant

Protein Characteristics: AA 1-1396

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This Cyclin B3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MPPPLLPKRS KLETEKAQSN KITPREEQQS EKIGKSNHAA SSSSSSTQGA VKRRSVFEDV TNASHSQCVQ SKEDNIELKS HVSKRTKKGV GEVTQKKIKS SKMGHVTSLS NMEKEFILDI PNKPKTLTTE EPSVFQKTLV LNEEPATKET CLMRKTLKSC AFHQETLLME KPLTLLVETE DYNEFDTELM TSKKKDKPED PTIIEEMTDL KKSVIRKVTL TSSPLWLKNK HVVQEEKPVI QEKSSFKRIS LVSNVVTTKE KPPVKKPHFR KKKPTTEMKS LLQEPSLEEK YNTQEDASIL KKPQVLQENT NNKDATLTEP VTFKGKHSAN EATHTKKPSS SKNNPDPQGK GTNLRPLRVH PVTYENEPMS SKKSTTKKKD SHFHGPSVLP DKHSPQMEVS TVKKSLALPN PTTEEKMLHF PVATVLEKQH NMGEAPCLKK PSPLRKQQQL PKRRRFFSNS AVQETVIRKP LFFKMSTTEK DPPSQWPSAL PKKHISPGEL SKQKKQHVSP KHNMEEDSQC WLDSAFKKQL SREEPASTHT PLKLEMQQAI TKETGFHLRN PLVLPTVTSE AKSLTKEPPS FREQNTSLLK RKSTTHTITL QQAQSEWQEM TDEDRNLFSI KPGSHRKEPI PEFLQNPLPP NENCLISQKL SHSMPFASQK TTSQERAHRK ESVASNDDKN FFSQDLFSPF SSADEDTLKF HKSLDFQEQV DRKNDSHKKM FDSQDSVSEE ESFLRKLFCK DRCSSTEELS QERTVALEQE FLLIKILNEN TSSDVDEPLS HQSPHIQNHS DTTKEALEAS EALEAPEALE TLEALVASED LEEPLNILEE LSTENMVALM KMLVTEDEST KDSFSGNYTA AREAHAEKSL SLEETSINEA ATLKESLSSQ EKHRAELVTV LKELLVLMKN PSLKRVALAF QENPSNNVET LLREVLALVE NSTADESTLQ EKPSTKTDVT PKELLALEEN SSNKKANPMD SLSFDHKPDT EMGEIARMVL TDEEYNIDTL YERVLALSQG LIAADQLSFT DLQNFEETKI VDEEEFFKSF LVFENKNSPN MSSNAFESRT DNSSAIMPSS KAFNPVENSN PYVSSSKSFK STLGAKETEI TIQDDSDSLE RIEKEGQDPL LNTIYAKDVF NYLKEREEKF LVQKYMDGQM ELTSDMRAIL VDWLVEIQGS FQMTHETLYL AVKIMDLYLM KAQCKKNHLQ LLGSTTYMIA AKFEESYPPS LSEFLFICED MYEKSDMVSL ESSILQTLNF DINIPTAYNF LRRYASCIHA SMKTLTLSRF ICEMTLQEYE YIEERPSKLA AASFILALYM RNLSNCVPTL EYFTGYKMAE LHILVRKLNH LLNFRSHSIL KNVFEKYSEE TYFEVAKIPP LSKQDLENLL NCALFH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Cyclin B3 (CCNB3)

Alternative Name: Ccnb3 (CCNB3 Products)

Synonyms: CG5814 Protein, Cyc B3 Protein, Dmel\\CG5814 Protein, anon-WO0118547.414 Protein, cycB3 Protein, l(3)L6540 Protein, ccnb3-a Protein, zgc:153369 Protein, MGC154490 Protein, NV16541 Protein, CCNB3 Protein, CYCB3 Protein, RGD1564367 Protein, Cyclin B3 Protein, cyclin B3 S homeolog Protein, cyclin B3 Protein, cyclin B3 L homeolog Protein, G2/mitotic-specific cyclin-B3 Protein, CycB3 Protein, ccnb3.S Protein, ccnb3 Protein, ccnb3.L Protein, LOC5572162 Protein, LOC100122822 Protein, CCNB3 Protein, LOC100394742 Protein, Ccnb3 Protein

Background: G2/mitotic-specific cyclin-B3,FUNCTION: Cyclins are positive regulatory subunits of the cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I (By similarity). {ECO:0000250}.

Molecular Weight: 159.0 kDa

UniProt: Q810T2

Pathways: Cell Division Cycle, Chromatin Binding