NLRP6 Protein (AA 1-869) (Strep Tag)

Catalog No. ABIN3154440

Product Details

Target: NLRP6 (NLR Family, Pyrin Domain Containing 6 (NLRP6))

Protein Type: Recombinant

Protein Characteristics: AA 1-869

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NLRP6 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MDAAGASCSS VDAVARELLM ATLEELSQEQ LKRFRHKLRD APLDGRSIPW GRLERSDAVD LVDKLIEFYE PVPAVEMTRQ VLKRSDIRDV ASRLKQQQLQ KLGPTSVLLS VSAFKKKYRE HVLRQHAKVK ERNARSVKIN KRFTKLLIAP GTGAVEDELL GPLGEPEPER ARRSDTHTFN RLFRGNDEES SQPLTVVLQG PAGIGKTMAA KKILYDWAAG KLYHSQVDFA FFMPCGELLE RPGKRSLADL VLDQCPDRAW PVKRILAQPN RLLFILDGAD ELPTLPSSEA TPCKDPLEAT SGLRVLSGLL SQELLPGARL LVTTRHAATG RLQGRLCSPQ CAEIRGFSDK DKKKYFFKFF RDERKAERAY RFVKENETLF ALCFVPFVCW IVCTVLQQQL ELGRDLSRTS KTTTSVYLLF ITSMLKSAGT NGPRVQGELR TLCRLAREGI LDHHKAQFSE EDLEKLKLRG SQVQTIFLNK KEIPGVLKTE VTYQFIDQSF QEFLAALSYL LEAERTPGTP AGGVQKLLNS DAELRGHLAL TTRFLFGLLN TEGLRDIGNH FGCVVPDHVK KDTLRWVQGQ SHPKGPPVGA KKTAELEDIE DAEEEEEEEE DLNFGLELLY CLYETQEEDF VRQALSSLPE IVLERVRLTR MDLEVLNYCV QCCPDGQALR LVSCGLVAAK EKKKKKKSLV KRLKGSQSTK KQPPVSLLRP LCETMTTPKC HLSVLILSHC RLPDAVCRDL SEALKVAPAL RELGLLQSRL TNTGLRLLCE GLAWPKCQVK TLRMQLPDLQ EVINYLVIVL QQSPVLTTLD LSGCQLPGVI VEPLCAALKH PKCSLKTLSL TSVELSENSL RDLQAVKTSK PDLSIIYSK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NLRP6 (NLR Family, Pyrin Domain Containing 6 (NLRP6))

Alternative Name: Nlrp6 (NLRP6 Products)

Synonyms: NLRP6 Protein, NALP6 Protein, AVR Protein, CLR11.4 Protein, NAVR Protein, NAVR/AVR Protein, PAN3 Protein, PYPAF5 Protein, AI504961 Protein, Avr Protein, Nalp6 Protein, Navr Protein, Navr/Avr Protein, Pypaf5 Protein, NLR family pyrin domain containing 6 Protein, NLR family, pyrin domain containing 6 Protein, NLRP6 Protein, Nlrp6 Protein

Background: NACHT, LRR and PYD domains-containing protein 6 (Angiotensin II/vasopressin receptor) (Non-angiotensin-vasopressin receptor) (Non-AVR) (PYRIN-containing APAF1-like protein 5-like),FUNCTION: Acts as the sensor component of the NLRP6 inflammasome, which mediates inflammasome activation in response to various pathogen-associated signals, leading to maturation and secretion of IL1B and IL18 (PubMed:21593405, PubMed:30392956, PubMed:32424362, PubMed:34678144). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (PubMed:30392956). Acts as a recognition receptor (PRR): recognizes and binds specific pathogens and other damage-associated signals, such as lipoteichoic acid (LTA), a cell-wall component of Gram-positive bacteria, or double stranded RNA (dsRNA) (PubMed:26494172, PubMed:30392956, PubMed:34678144). May also recognize and bind lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria, however, LPS is probably not a major activator of the NLRP6 inflammasome (PubMed:34678144). Following LTA- or dsRNA-binding, NLRP6 undergoes liquid-liquid phase separation (LLPS), enhancing multivalent interactions, an essential step for the formation of the NLRP6 inflammasome polymeric complex (PubMed:34678144). The NLRP6 inflammasome acts by promoting recruitment of effector pro-inflammatory caspases (CASP1 and/or CASP4) that catalyze maturation and secretion of IL1B and IL18 in the extracellular milieu (PubMed:30392956). The NLRP6 inflammasome plays a central role in the maintenance of epithelial integrity and host defense against microbial infections in the intestine (PubMed:21565393, PubMed:22763455, PubMed:23696660, PubMed:26638072, PubMed:28445725, PubMed:30392956). Required to restrict infection against Gram-positive bacteria by recognizing lipoteichoic acid (LTA), leading to recruitment of CASP4 and CASP1, and subsequent maturation and secretion of IL1B and IL18 (PubMed:30392956). Involved in intestinal antiviral innate immunity together with DHX15: recognizes and binds viral dsRNA to restrict infection by enteric viruses through the interferon pathway and GSDMD-dependent release of IL18 (PubMed:26494172, PubMed:34678144). Required to prevent infection by the apicomplexan parasite C.tyzzeri in enterocytes by promoting GSDMD-dependent release of IL18 (PubMed:33372132). The NLRP6 inflammasome may also regulate the gut microbiota composition by acting as a sensor of microbiota-associated metabolites to form a PYCARD/ASC-dependent inflammasome for downstream IL18 release and secretion of antimicrobial peptides (PubMed:21565393, PubMed:22763455, PubMed:26638072, PubMed:33617596). Its role in the regulation of the gut microbiota composition is however subject to discussion (PubMed:29281815, PubMed:29281837, PubMed:28801232). Essential for gut mucosal self-renewal and proliferation (PubMed:21593405, PubMed:21543645, PubMed:21565393). Regulate mucus secretion in an inflammasome- and autophagy-dependent manner to prevent invasion by enteric bacteria (PubMed:24581500, PubMed:27339979). During systemic bacterial infections, the NLRP6 inflammasome negatively regulates neutrophil recruitment and neutrophil extracellular traps (NETs) formation (PubMed:22763455, PubMed:30248149, PubMed:33918100, PubMed:33230225). May promote peripheral nerve recovery following injury via an inflammasome-independent mechanism (PubMed:26253422). {ECO:0000269|PubMed:21543645, ECO:0000269|PubMed:21565393, ECO:0000269|PubMed:21593405, ECO:0000269|PubMed:22763455, ECO:0000269|PubMed:23696660, ECO:0000269|PubMed:24581500, ECO:0000269|PubMed:26253422, ECO:0000269|PubMed:26494172, ECO:0000269|PubMed:26638072, ECO:0000269|PubMed:27339979, ECO:0000269|PubMed:28445725, ECO:0000269|PubMed:28801232, ECO:0000269|PubMed:29281815, ECO:0000269|PubMed:29281837, ECO:0000269|PubMed:30248149, ECO:0000269|PubMed:30392956, ECO:0000269|PubMed:32424362, ECO:0000269|PubMed:33230225, ECO:0000269|PubMed:33372132, ECO:0000269|PubMed:33617596, ECO:0000269|PubMed:33918100, ECO:0000269|PubMed:34678144}.

Molecular Weight: 97.4 kDa

UniProt: Q91WS2

Pathways: Inflammasome