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Sortilin 1 Protein (SORT1) (AA 74-825) (rho-1D4 tag)

Crystallography grade SORT1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3135820
$26,631.34
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
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  • Target See all Sortilin 1 (SORT1) Proteins
    Sortilin 1 (SORT1)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 74-825
    Origin
    • 7
    • 2
    • 2
    Mouse
    Source
    • 4
    • 3
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Sortilin 1 protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    RRGAPAEDQD CGRLPDFIAK LTNNTHQHVF DDLSGSVSLS WVGDSTGVIL VLTTFQVPLV IVSFGQSKLY RSEDYGKNFK DITNLINNTF IRTEFGMAIG PENSGKVILT AEVSGGSRGG RVFRSSDFAK NFVQTDLPFH PLTQMMYSPQ NSDYLLALST ENGLWVSKNF GEKWEEIHKA VCLAKWGPNN IIFFTTHVNG SCKADLGALE LWRTSDLGKT FKTIGVKIYS FGLGGRFLFA SVMADKDTTR RIHVSTDQGD TWSMAQLPSV GQEQFYSILA ANEDMVFMHV DEPGDTGFGT IFTSDDRGIV YSKSLDRHLY TTTGGETDFT NVTSLRGVYI TSTLSEDNSI QSMITFDQGG RWEHLRKPEN SKCDATAKNK NECSLHIHAS YSISQKLNVP MAPLSEPNAV GIVIAHGSVG DAISVMVPDV YISDDGGYSW AKMLEGPHYY TILDSGGIIV AIEHSNRPIN VIKFSTDEGQ CWQSYVFTQE PIYFTGLASE PGARSMNISI WGFTESFITR QWVSYTVDFK DILERNCEED DYTTWLAHST DPGDYKDGCI LGYKEQFLRL RKSSVCQNGR DYVVAKQPSV CPCSLEDFLC DFGYFRPENA SECVEQPELK GHELEFCLYG KEEHLTTNGY RKIPGDKCQG GMNPAREVKD LKKKCTSNFL NPTKQNSKSN SVPIILAIVG LMLVTVVAGV LIVKKYVCGG RFLVHRYSVL QQHAEADGVE ALDSTSHAKS GYHDDSDEDL LE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Sort1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Sortilin 1 (SORT1)
    Alternative Name
    Sort1 (SORT1 Products)
    Synonyms
    SORT1 Protein, 19.m02079 Protein, gp95 Protein, ldlcq6 Protein, sortilin Protein, Gp95 Protein, LDLCQ6 Protein, NT3 Protein, 2900053A11Rik Protein, AI852375 Protein, Ntr3 Protein, Ntsr3 Protein, Nt3 Protein, Nts3 Protein, sort1 Protein, zgc:85953 Protein, sortilin 1 Protein, sortilin Protein, sortilin 1 S homeolog Protein, sortilin 1a Protein, SORT1 Protein, TP01_0016 Protein, BBOV_I002730 Protein, sort1.S Protein, Sort1 Protein, sort1a Protein
    Background
    Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. {ECO:0000269|PubMed:10594043, ECO:0000269|PubMed:15236332, ECO:0000269|PubMed:15236333, ECO:0000269|PubMed:15372498, ECO:0000269|PubMed:15992544, ECO:0000269|PubMed:19407813, ECO:0000269|PubMed:21102451}.
    Molecular Weight
    84.7 kDa Including tag.
    UniProt
    Q6PHU5
    Pathways
    Neurotrophin Signaling Pathway
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