PRIM1 Protein (AA 1-420) (GST tag)
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- Target See all PRIM1 Proteins
- PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-420
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PRIM1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PRIM1 (Human) Recombinant Protein (P01)
- Sequence
- METFDPTELP ELLKLYYRRL FPYSQYYRWL NYGGVIKNYF QHREFSFTLK DDIYIRYQSF NNQSDLEKEM QKMNPYKIDI GAVYSHRPNQ HNTVKLGAFQ AQEKELVFDI DMTDYDDVRR CRSSADICPK CWTLMTMAIR IIDRALKEDF GFKHRLWVYS GRRGVHCWVC DESVRKLSSA VRSGIVEYLS LVKGGQDVKK KVHLSEKIHP FIRKSINIIK KYFEEYALVN QDILENKESW DKILALVPET IHDELQQSFQ KSHNSLQRWE HLKKVASRYQ NNIKNDKYGP WLEWEIMLQY CFPRLDINVS KGINHLLKSP FSVHPKTGRI SVPIDLQKVD QFDPFTVPTI SFICRELDAI STNEEEKEEN EAESDVKHRT RDYKKTSLAP YVKVFEHFLE NLDKSRKGEL LKKSDLQKDF
- Characteristics
- Human PRIM1 full-length ORF (BAG37381.1, 1 a.a. - 420 a.a.) recombinant protein with GST tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))
- Alternative Name
- PRIM1 (PRIM1 Products)
- Synonyms
- AI324982 Protein, p49 Protein, dnapol-alpha50 Protein, DNA primase, p49 subunit Protein, DNA primase subunit 1 Protein, DNA primase subunit 1 S homeolog Protein, Prim1 Protein, PRIM1 Protein, prim1.S Protein
- Background
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Synonyms: MGC12308,p49
Gene Description: primase, DNA, polypeptide 1 (49kDa)
Gene Name: PRIM1
Gene Summary: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
GenBank: AK314866.1, BAG37381.1 - Molecular Weight
- 72.6 kDa (theoreatical)
- Gene ID
- 5557
- Pathways
- Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, SARS-CoV-2 Protein Interactome
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