Ubiquitin B Protein (UBB) (AA 153-228) (GST tag,His tag)

Catalog No. ABIN7313880

Product Details

Target: Ubiquitin B (UBB)

Protein Type: Recombinant

Protein Characteristics: AA 153-228

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Ubiquitin B protein is labelled with GST tag,His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)

Purpose: Recombinant Human UBB Protein is produced by our E. coli expression system and the target gene encoding Met153-Gly228 is expressed with a GST, 6His tag at the N-terminus.

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKSPM GYRGSMQIFV KTLTGKTITL EVEPSDTIEN VKAKIQDKEG IPPDQQRLIF AGKQLEDGRT LSDYNIQKES TLHLVLRLRG G

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: Ubiquitin B (UBB)

Alternative Name: UBB (UBB Products)

Synonyms: AL033289 Protein, Rps27a Protein, Uba52 Protein, Ubb2 Protein, Ubc Protein, UBC Protein, Ubiquitin Protein, UBB Protein, Ubi-p63E Protein, im:6892314 Protein, si:ch211-202a12.3 Protein, ubc Protein, zUBC Protein, zgc:172187 Protein, ubiquitin B Protein, ubiquitin C Protein, UBB Protein, Ubb Protein, UBC Protein, ubb Protein

Gene ID: 7314

UniProt: P0CG47

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway