TRIM5 Protein (AA 1-248) (His tag)
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- Target See all TRIM5 Proteins
- TRIM5 (Tripartite Motif Containing 5 (TRIM5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-248
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TRIM5 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human TRIM5 Protein is produced by our E. coli expression system and the target gene encoding Met1-Gln248 is expressed with a 6His tag at the N-terminus.
- Sequence
- MGSSHHHHHH SSGLVPRGSH MASGILVNVK EEVTCPICLE LLTQPLSLDC GHSFCQACLT ANHKKSMLDK GESSCPVCRI SYQPENIRPN RHVANIVEKL REVKLSPEGQ KVDHCARHGE KLLLFCQEDG KVICWLCERS QEHRGHHTFL TEEVAREYQV KLQAALEMLR QKQQEAEELE ADIREEKASW KTQIQYDKTN VLADFEQLRD ILDWEESNEL QNLEKEEEDI LKSLTNSETE MVQQTQSLRE LISDLEHRLQ GSVMELLQ
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- TRIM5 (Tripartite Motif Containing 5 (TRIM5))
- Alternative Name
- TRIM5 (TRIM5 Products)
- Synonyms
- TRIM5 Protein, RNF88 Protein, TRIM5alpha Protein, EG667823 Protein, Gm8833 Protein, RGD1304579 Protein, tripartite motif containing 5 Protein, tripartite motif-containing 5 Protein, TRIM5 Protein, Trim5 Protein
- Gene ID
- 85363
- UniProt
- Q9C035
- Pathways
- Activation of Innate immune Response
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