AIF Protein (AA 121-613) (His tag)

Catalog No. ABIN7311234

Product Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Protein Type: Recombinant

Protein Characteristics: AA 121-613

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This AIF protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human AIFM1 Protein is produced by our E. coli expression system and the target gene encoding Glu121-Asp613 is expressed with a 6His tag at the N-terminus.

Sequence: MGSSHHHHHH SSGLVPRGSH MEEVPQDKAP SHVPFLLIGG GTAAFAAARS IRARDPGARV LIVSEDPELP YMRPPLSKEL WFSDDPNVTK TLRFKQWNGK ERSIYFQPPS FYVSAQDLPH IENGGVAVLT GKKVVQLDVR DNMVKLNDGS QITYEKCLIA TGGTPRSLSA IDRAGAEVKS RTTLFRKIGD FRSLEKISRE VKSITIIGGG FLGSELACAL GRKARALGTE VIQLFPEKGN MGKILPEYLS NWTMEKVRRE GVKVMPNAIV QSVGVSSGKL LIKLKDGRKV ETDHIVAAVG LEPNVELAKT GGLEIDSDFG GFRVNAELQA RSNIWVAGDA ACFYDIKLGR RRVEHHDHAV VSGRLAGENM TGAAKPYWHQ SMFWSDLGPD VGYEAIGLVD SSLPTVGVFA KATAQDNPKS ATEQSGTGIR SESETESEAS EITIPPSTPA VPQAPVQGED YGKGVIFYLR DKVVVGIVLW NIFNRMPIAR KIIKDGEQHE DLNEVAKLFN IHED

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1 (AIFM1 Products)

Synonyms: AIF Protein, CMTX4 Protein, COWCK Protein, COXPD6 Protein, PDCD8 Protein, CG7263 Protein, DmAIF Protein, Dmel\\CG7263 Protein, GB16024 Protein, DDBDRAFT_0187853 Protein, DDBDRAFT_0191137 Protein, DDB_0187853 Protein, DDB_0191137 Protein, aif Protein, pdcd8 Protein, AIFM1 Protein, PCD8 Protein, AIFsh2 Protein, Hq Protein, Pdcd8 Protein, mAIF Protein, Aif Protein, zgc:91994 Protein, apoptosis inducing factor mitochondria associated 1 Protein, allograft inflammatory factor 1 Protein, Apoptosis inducing factor Protein, apoptosis-inducing factor 1, mitochondrial Protein, apoptosis inducing factor Protein, apoptosis inducing factor, mitochondria associated 1 Protein, apoptosis-inducing factor, mitochondrion-associated, 1 Protein, apoptosis-inducing factor, mitochondrion-associated 1 Protein, AIFM1 Protein, AIF1 Protein, AIF Protein, LOC412212 Protein, aif Protein, aifm1 Protein, Aifm1 Protein

Gene ID: 9131

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect