Caspase 10 Protein (AA 220-480) (His tag)
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- Target See all Caspase 10 (CASP10) Proteins
- Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 220-480
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Caspase 10 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human CASP10 Protein is produced by our E. coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.
- Sequence
- MVKTFLEALP QESWQNKHAG SNGNRATNGA PSLVSRGMQG ASANTLNSET STKRAAVYRM NRNHRGLCVI VNNHSFTSLK DRQGTHKDAE ILSHVFQWLG FTVHIHNNVT KVEMEMVLQK QKCNPAHADG DCFVFCILTH GRFGAVYSSD EALIPIREIM SHFTALQCPR LAEKPKLFFI QACQGEEIQP SVSIEADALN PEQAPTSLQD SIPAEADFLL GLATVPGYVS FRHVEEGSWY IQSLCNHLKK LVPRHEDILS ILEHHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 25 mM HEPES, 10 mM DTT, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))
- Alternative Name
- CASP10 (CASP10 Products)
- Synonyms
- xCaspase-10 Protein, alps2 Protein, caspace-10 Protein, caspase-10 Protein, flice2 Protein, mch4 Protein, xCaspace-10 Protein, ALPS2 Protein, FLICE2 Protein, MCH4 Protein, caspase 10 S homeolog Protein, caspase 10 Protein, caspase-10 Protein, casp10.S Protein, casp10 Protein, CASP10 Protein, Casp10 Protein, LOC100848685 Protein
- Gene ID
- 843
- UniProt
- Q92851
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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