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IL12A & IL27B (AA 20-189), (AA 23-328) protein (His tag)

Origin: Human Host: Human Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. ELISA, MS, SDS, WB
Catalog No. ABIN7312620
  • Target
    IL12A & IL27B
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-328, AA 20-189
    Origin
    • 4
    • 3
    • 1
    • 1
    Human
    Source
    • 6
    Human Cells
    Purification tag / Conjugate
    His tag
    Application
    ELISA, Mass Spectrometry (MS), SDS-PAGE (SDS), Western Blotting (WB)
    Purpose
    Recombinant Human IL23A/IL12B Protein is produced by our mammalian expression system and the target gene encoding Arg20-Pro189&Ile23-Ser328 is expressed with a 6His tag at the C-terminus.
    Sequence
    RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP VDHHHHHH&I WELKKDVYVV ELDWYPDAPG EMVVLTCDTP EEDGITWTLD QSSEVLGSGK TLTIQVKEFG DAGQYTCHKG GEVLSHSLLL LHKKEDGIWS TDILKDQKEP KNKTFLRCEA KNYSGRFTCW WLTTISTDLT FSVKSSRGSS DPQGVTCGAA TLSAERVRGD NKEYEYSVEC QEDSACPAAE ESLPIEVMVD AVHKLKYENY TSSFFIRDII KPDPPKNLQL KPLKNSRQVE VSWEYPDTWS TPHSYFSLTF CVQVQGKSKR EKKDRVFTDK TSATVICRKN ASISVRAQDR YYSSSWSEWA SVPCS
    Purity
    Greater than 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Lyophilized from a 0.2 μM filtered solution of PBS pH 7.4.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
  • Target
    IL12A & IL27B
    Alternative Name
    IL23A, IL12B
    Gene ID
    51561, 3593
    UniProt
    Q9NPF7, P29460
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