KIT Ligand (KITLG) (AA 26-190) (Active) protein (AVI tag,Biotin,His tag)

Details for Product No. ABIN4949036, Supplier: Log in to see
Protein Name
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
  • steel
  • KITLG
  • SCF
  • kitl
  • kl-1
  • mgf
  • scf
  • FPH2
  • KL-1
  • Kitl
  • MGF
  • SF
  • SHEP7
  • Clo
  • Con
  • Gb
  • Kitlg
  • Mgf
  • SLF
  • Sl
  • blz
  • contrasted
  • 710-712
  • CSF
  • KITL
  • KIT ligand L homeolog
  • KIT ligand
  • kit ligand
  • kitlg.L
  • KITLG
  • kitlg
  • Kitl
  • Kitlg
Protein Characteristics
AA 26-190
21
6
6
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Origin
Human
69
49
21
4
2
2
2
2
2
1
1
1
Source
HEK-293 Cells
101
13
9
8
3
3
3
2
2
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This KIT Ligand protein is labelled with AVI tag,Biotin,His tag.
Application
Functional Studies (Func)
Options
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Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
Molecular Weight 21.7 kDa
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for KIT Ligand (KITLG) (AA 26-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949036) Biotinylated Human SCF, His Tag on SDS-PAGE under reducing (R) condition. The gel was...
 image for KIT Ligand (KITLG) (AA 26-190) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949036) Measured by its binding ability in a functional ELISA. Immobilized Human CD117, Fc Ta...