Cyclin A1 Protein (CCNA1) (GST tag,His tag)
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- Target See all Cyclin A1 (CCNA1) Proteins
- Cyclin A1 (CCNA1)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Cyclin A1 protein is labelled with GST tag,His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Purified recombinant protein of Human cyclin A1 (CCNA1), transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50 μg (full length, N-term GST tag, C-term His tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purification
- Purified
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product CCNA1 Protein
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Cyclin A1 (CCNA1) (AA 18-172) protein (His tag)
CCNA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin A1 (CCNA1) (AA 1-464) protein (GST tag)CCNA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin A1 (CCNA1) (AA 1-465) protein (Strep Tag)Crystallography grade CCNA1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cyclin A1 (CCNA1) (AA 1-421) protein (Strep Tag)Crystallography grade CCNA1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cyclin A1 (CCNA1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CCNA1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal,N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Cyclin A1 (CCNA1)
- Alternative Name
- cyclin A1 (CCNA1 Products)
- Synonyms
- fd14f08 Protein, wu:fd14f08 Protein, wu:fi20d12 Protein, zgc:111869 Protein, MGC81965 Protein, cyclin-A1 Protein, CCNA1 Protein, MGC132235 Protein, cyclin-A1-2 Protein, Cyclin-A1 Protein, ccna1 Protein, cyclin A1 Protein, cyclin A1 L homeolog Protein, CYCLIN A1;2 Protein, cyclin A1 S homeolog Protein, CCNA1 Protein, ccna1 Protein, ccna1.L Protein, CYCA1;2 Protein, Ccna1 Protein, ccna1.S Protein
- Background
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 78.2 kDa
- NCBI Accession
- NP_003905
- Pathways
- Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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