Cyclin A1 Protein (CCNA1) (His tag)
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- Target See all Cyclin A1 (CCNA1) Proteins
- Cyclin A1 (CCNA1)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Cyclin A1 protein is labelled with His tag.
- Purpose
- Recombinant Human CCNA1/Cyclin-A1 Protein (His Tag)(Active)
- Sequence
- Met 1-Gln 465
- Characteristics
- A DNA sequence encoding the full length of human CCNA1 isoform a (NP_003905.1) (Met 1-Gln 465) was expressed, with a polyhistidine tag at the N-terminus.
- Purity
- > 96 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- 1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml.Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml.
- Top Product
- Discover our top product CCNA1 Protein
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Cyclin A1 (CCNA1) (AA 18-172) protein (His tag)
CCNA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin A1 (CCNA1) (AA 1-464) protein (GST tag)CCNA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin A1 (CCNA1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CCNA1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Cyclin A1 (CCNA1)
- Alternative Name
- CCNA1/Cyclin-A1 (CCNA1 Products)
- Synonyms
- fd14f08 Protein, wu:fd14f08 Protein, wu:fi20d12 Protein, zgc:111869 Protein, MGC81965 Protein, cyclin-A1 Protein, CCNA1 Protein, MGC132235 Protein, cyclin-A1-2 Protein, Cyclin-A1 Protein, ccna1 Protein, cyclin A1 Protein, cyclin A1 L homeolog Protein, CYCLIN A1;2 Protein, cyclin A1 S homeolog Protein, CCNA1 Protein, ccna1 Protein, ccna1.L Protein, CYCA1;2 Protein, Ccna1 Protein, ccna1.S Protein
- Background
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Background: Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.
Synonym: CT146
- Molecular Weight
- 54.6 kDa
- NCBI Accession
- NP_003905
- Pathways
- Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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