IGFBP6 Protein
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- Target See all IGFBP6 Proteins
- IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Hi-5 Cells
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human IGFBP6 protein expressed in Hi-5 insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to inhibit IGF-II induced proliferation of human MCF-7 cells. The expected ED50 for this effect is 0.1 - 0.4ug/mL.
- Top Product
- Discover our top product IGFBP6 Protein
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Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) protein (His tag)
IGFBP6 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) (AA 28-240) protein (His tag)IGFBP6 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) (AA 1-240) protein (GST tag)IGFBP6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) (Active) protein (His tag)IGFBP6 Origin: Mouse Host: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) protein (His tag)IGFBP6 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
- Alternative Name
- IGFBP6 (IGFBP6 Products)
- Synonyms
- IBP6 Protein, IGFBP-6 Protein, IGF-BP6 Protein, RBP6A Protein, IGFBP6 Protein, igfbp6 Protein, insulin like growth factor binding protein 6 Protein, insulin-like growth factor binding protein 6 Protein, IGFBP6 Protein, Igfbp6 Protein, IGFBP-6 Protein, igfbp6 Protein
- Background
- IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 22.6 kDa
- NCBI Accession
- NP_002169
- Pathways
- WNT Signaling, Myometrial Relaxation and Contraction
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