SGK1 Protein (Ser422Asp-Mutant) (GST tag)
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- Target See all SGK1 Proteins
- SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Ser422Asp-Mutant
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Origin
- Human
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Source
- Insect cells (Sf21)
- Purification tag / Conjugate
- This SGK1 protein is labelled with GST tag.
- Application
- SDS-PAGE (SDS), Functional Studies (Func)
- Purpose
- SGK1 (S422D) (Human) Recombinant Protein
- Characteristics
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Human SGK1 (NP_005618.2, 61 a.a. - 431 a.a.) S422D mutant partial recombinant protein with GST tag co-expressed with His-tagged PDPK1 (NP_002604.1, 1 a.a. - 556 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
This product with activity data is belong to bioactive protein. - Purification
- Baculovirus infected insect cell (Sf21) expression system
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serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) (Active) protein (GST tag)
SGK1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 60-431) protein (His tag)SGK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) protein (GST tag)SGK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 113-252) protein (GST tag)SGK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) protein (GST tag)SGK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) protein (Strep Tag)Crystallography grade SGK1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) protein (Strep Tag)Crystallography grade SGK1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
serum/glucocorticoid Regulated Kinase 1 (SGK1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SGK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Baculovirus infected insect cell (Sf21) expression system
Product Quality tested by: SDS-PAGE Stained with Coomassie Blue - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05 % Brij35, 1 mM DTT, 10 % glycerol)
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
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- Target
- SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
- Alternative Name
- SGK1 (SGK1 Products)
- Synonyms
- SGK Protein, Sgk Protein, sgk-B Protein, sgk2 Protein, cb1083 Protein, sgk Protein, wu:fc20a09 Protein, sgk-A Protein, sgk1 Protein, sgk1-a Protein, sgk1-b Protein, serum/glucocorticoid regulated kinase 1 Protein, Serine/threonine-protein kinase sgk-1 Protein, serum/glucocorticoid regulated kinase 1 S homeolog Protein, serum/glucocorticoid regulated kinase 1 L homeolog Protein, SGK1 Protein, Sgk1 Protein, sgk-1 Protein, sgk1.S Protein, sgk1 Protein, sgk1.L Protein
- Background
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Full Gene Name: serum/glucocorticoid regulated kinase 1
Synonyms: SGK - Gene ID
- 6446
- Pathways
- MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway
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