CD276 Protein (CD276) (AA 47-219) (His tag)

Catalog No. ABIN5665775

Product Details

Target: CD276

Biological Activity: Active

Protein Characteristics: AA 47-219

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CD276 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 97 %

Biological Activity Comment: Cluster Of Differentiation 276 (CD276) also known as B7-H3 in human, is a member of the B7/CD28 superfamily of costimulatory molecules serving as an accessory modulator of T-cell response. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production. Besides, Sialic acid-binding Ig-like lectin 12 (SIGLEC12) has been identified as an interactor of CD276, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD276 and recombinant human SIGLEC12. Briefly, CD276 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC12-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD276 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CD276 and SIGLEC12 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CD276 with SIGLEC12.

Application Details

Application Notes: Isoelectric Point: 5.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: CD276

Alternative Name: Cluster Of Differentiation 276 (CD276) (CD276 Products)

Synonyms: sc:d0645 Protein, zgc:158279 Protein, 6030411F23Rik Protein, AU016588 Protein, B7RP-2 Protein, B7h3 Protein, B7H3 Protein, 4Ig-B7-H3 Protein, B7-H3 Protein, CD276 molecule Protein, CD276 molecule S homeolog Protein, CD276 antigen Protein, Cd276 molecule Protein, CD276 Protein, cd276.S Protein, cd276 Protein, Cd276 Protein

Background: Alternative Names: B7-H3, B7H3, CD276 Molecule, B7 homolog 3, Costimulatory molecule

Molecular Weight: 22kDa

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints