IL17C Protein (AA 36-187) (His tag)
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- Target See all IL17C Proteins
- IL17C (Interleukin 17C (IL17C))
- Biological Activity
- Active
- Protein Characteristics
- AA 36-187
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL17C protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to plays a crucial role in innate immunity of the epithelium. IL17C binds to IL17RA with affinity. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17C with recombinant human IL17RA. Briefly, IL17C were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17C with IL17RA.
- Top Product
- Discover our top product IL17C Protein
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Interleukin 17C (IL17C) (AA 19-197) protein (His-Avi Tag)
IL17C Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Interleukin 17C (IL17C) (AA 1-197) protein (GST tag)IL17C Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 17C (IL17C) (AA 19-197) protein (His tag)Crystallography grade IL17C Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 17C (IL17C) (AA 17-194) protein (His tag)Crystallography grade IL17C Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 17C (IL17C) (AA 19-197) (Active) protein (His tag)IL17C Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 17C (IL17C) (AA 19-197) (Active) protein (His tag,AVI tag,Biotin)IL17C Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Application Notes
- Isoelectric Point: 8.6
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL17C (Interleukin 17C (IL17C))
- Abstract
- IL17C Products
- Synonyms
- IL17C Protein, il17-3 Protein, CX2 Protein, IL-17C Protein, IL-21 Protein, interleukin 17C Protein, interleukin 17c Protein, IL17C Protein, il17c Protein, Il17c Protein
- Background
- Alternative Names: Cytokine CX2
- Molecular Weight
- 19kDa
- UniProt
- Q9P0M4
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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