JAK3 Protein (AA 818-110) (His tag)
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- Target See all JAK3 Proteins
- JAK3 (Janus Kinase 3 (JAK3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 818-110
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This JAK3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- LKYISLLGKG NFGSVELCRY DPLGDNTGPL VAVKQLQHSG PDQQRDFQRE IQILKALHSD FIVKYRGVSY GPGRQSLRLV MEYLPSGCLR DFLQRHRARL HTDRLLLFAW QICKGMEYLG ARRCVHRDLA ARNILVESEA HVKIADFGLA KLLPLGKDYY VVREPGQSPI FWYAPESLSD NIFSRQSDVW SFGVVLYELF TYCDKSCSPS AEFLRMMGPE REGPPLCRLL ELLAEGRRLP PPPTCPTEVQ ELMQLCWAPS PHDRPAFGTL SPQLDALWRG RPG
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product JAK3 Protein
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Janus Kinase 3 (JAK3) (AA 795-1124) (Active) protein (GST tag)
JAK3 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
Janus Kinase 3 (JAK3) (AA 781-1124) (Active) protein (His-GST)JAK3 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Janus Kinase 3 (JAK3) (AA 1-619) protein (GST tag)JAK3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Janus Kinase 3 (JAK3) (AA 1-1124) protein (Strep Tag)Crystallography grade JAK3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Janus Kinase 3 (JAK3) (AA 1-1100) protein (Strep Tag)Crystallography grade JAK3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Janus Kinase 3 (JAK3) (AA 818-110) protein (His tag)JAK3 Origin: Mouse Host: Yeast Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- JAK3 (Janus Kinase 3 (JAK3))
- Alternative Name
- JAK3 (JAK3 Products)
- Synonyms
- JAK-3 Protein, JAK3_HUMAN Protein, JAKL Protein, L-JAK Protein, LJAK Protein, fae Protein, wil Protein, RATJAK3 Protein, JAK Protein, Janus kinase 3 Protein, JAK3 Protein, Jak3 Protein
- Background
- Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hatopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 Molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
- Molecular Weight
- 36.3 kDa
- UniProt
- Q62137
- Pathways
- JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response
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