NEIL1 Protein (AA 2-390) (His-SUMO Tag)
-
- Target See all NEIL1 Proteins
- NEIL1 (Endonuclease VIII-Like 1 (NEIL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-390
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NEIL1 protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- PEGPELHLAS QFVNEACRAL VFGGCVEKSS VSRNPEVPFE SSAYRISASA RGKELRLILS PLPGAQPQQE PLALVFRFGM SGSFQLVPRE ELPRHAHLRF YTAPPGPRLA LCFVDIRRFG RWDLGGKWQP GRGPCVLQEY QQFRENVLRN LADKAFDRPI CEALLDQRFF NGIGNYLRAE ILYRLKIPPF EKARSVLEAL QQHRPSPELT LSQKIRTKLQ NPDLLELCHS VPKEVVQLGG RGYGSESGEE DFAAFRAWLR CYGMPGMSSL QDRHGRTIWF QGDPGPLAPK GRKSRKKKSK ATQLSPEDRV EDALPPSKAP SRTRRAKRDL PKRTATQRPE GTSLQQDPEA PTVPKKGRRK GRQAASGHCR PRKVKADIPS LEPEGTSAS
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product NEIL1 Protein
-
Endonuclease VIII-Like 1 (NEIL1) (AA 200-390) protein (His tag)
NEIL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Endonuclease VIII-Like 1 (NEIL1) (AA 302-390) protein (GST tag)NEIL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Endonuclease VIII-Like 1 (NEIL1) (AA 1-390) protein (His tag)NEIL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Endonuclease VIII-Like 1 (NEIL1) (AA 1-390) protein (GST tag)NEIL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Endonuclease VIII-Like 1 (NEIL1) (AA 2-390) protein (His tag)Crystallography grade NEIL1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Endonuclease VIII-Like 1 (NEIL1) (AA 2-389) protein (His tag)Crystallography grade NEIL1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Endonuclease VIII-Like 1 (NEIL1) protein (Myc-DYKDDDDK Tag)NEIL1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
- Target
- NEIL1 (Endonuclease VIII-Like 1 (NEIL1))
- Alternative Name
- NEIL1 (NEIL1 Products)
- Synonyms
- FPG1 Protein, NEI1 Protein, hFPG1 Protein, 2810450N13Rik Protein, Nei1 Protein, nei-like DNA glycosylase 1 Protein, nei like DNA glycosylase 1 Protein, nei endonuclease VIII-like 1 (E. coli) Protein, Neil1 Protein, NEIL1 Protein
- Background
- Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and roves damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the roved base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
- Molecular Weight
- 59.6 kDa
- UniProt
- Q96FI4
- Pathways
- DNA Damage Repair
-
