VCAM1 Protein (AA 25-698) (His tag)
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- Target See all VCAM1 Proteins
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-698
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This VCAM1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPEFFKIET TPESRYLAQI GDSVSLTCST TGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDP EIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDI GKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWS KKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGD SVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVE LYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMT FIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGF PAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFP SESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLR SLTLDVQGRE NNKDYFSPEH HHHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates 10ug/ml. This effect is more to 70%.
- Top Product
- Discover our top product VCAM1 Protein
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Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-697) protein (His tag)
VCAM1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (Fc Tag)VCAM1 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-309) protein (His tag)VCAM1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) protein (GST tag)VCAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-123) protein (GST tag)VCAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag,Fc Tag)VCAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (His tag)VCAM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag)VCAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-698) protein (His tag)Crystallography grade VCAM1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-739) protein (rho-1D4 tag)Crystallography grade VCAM1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- Alternative Name
- VCAM1 (VCAM1 Products)
- Synonyms
- CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein
- Background
- VCAM1, also known as vascular cell adhesion protein 1 isoform, a member of the immunoglobulin superfamily. It is a cell surface protein expressed by activated endothelial cells and certain leukocytes. VCAM1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection and asthmatic responses. During the inflammatory adhesion mechanism, activated integrins that rolling leukocytes and attach them firmly to the vascular endothelium. Recombinant human VCAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 75.6kDa (685aa) 70-100KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_001069
- UniProt
- P19320
- Pathways
- Carbohydrate Homeostasis
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