ITGAV/ITGB5 Protein (AA 31-992) (His tag,AVI tag,Biotin)
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- Target See all ITGAV/ITGB5 products
- ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-992
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ITGAV/ITGB5 protein is labelled with His tag,AVI tag,Biotin.
- Sequence
- AA 31-992
- Specificity
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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- Comment
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 50 mM Tris, 150 mM NaCl, pH 7.5
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
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- Target
- ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))
- Alternative Name
- Integrin alpha V beta 5 (ITGAV/ITGB5 Products)
- Background
- Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.
- Molecular Weight
- 114.7 kDa (ITGAV) & 81.9 kDa (ITGB5)
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