ITGAV/ITGB5 Protein (AA 31-992) (His-Avi Tag,Biotin)
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- Target See all ITGAV/ITGB5 products
- ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-992
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ITGAV/ITGB5 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human Integrin alpha V beta 5 (ITGAV & ITGB5) Heterodimer Protein
- Sequence
- Phe31-Val992 (ITGAV) acidic tail & Gly24-Asn719 (ITGB5) basic tail
- Specificity
- Uni-Prot: P06756-1 (ITGAV), P18084 (ITGB5)
- Characteristics
- Recombinant Biotinylated Human Integrin alpha V beta 5 (ITGAV & ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-alpha V Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB5, His Tag with the EC50 of 0.29ug/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ITGAV/ITGB5 (Integrin alpha V / beta 5 (ITGAV/ITGB5))
- Alternative Name
- ITGAV&ITGB5 (ITGAV/ITGB5 Products)
- Background
- Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C.
- Molecular Weight
- 114 kDa (ITGAV)&81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.
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